A STAINING METHOD FOR BONE CANALICULI
A STAINING METHOD FOR BONE CANALICULI
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DOI：
10.3109/17453679508995514
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发表时间：
1995-04-01
期刊：
影响因子：
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通讯作者：
HIRASAWA, Y
中科院分区：
文献类型：
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作者：
KUSUZAKI, K;KAGEYAMA, N;HIRASAWA, Y
用丙氨酸银蛋白改良的Bodian法通常用于神经纤维的检测。将该技术应用于脱钙大鼠骨切片，骨小管被清晰地染成黑色，与板层骨和编织骨中的骨基质形成良好的对比。此外，骨小管与其他小管、成骨细胞、破骨细胞和软骨细胞之间的连接也很容易被发现。我们发现编织骨的骨小管比板层骨更少，排列更不规则，我们认为这种脱钙骨骨小管染色方法优于以往报道的方法，可能在骨病理研究中有一定的应用价值。
The modified Bodian method with protargol (silver protein) is ordinarily used to detect nerve fibers. With this technique, applied to decalcified rat bone sections, the bone canaliculi were clearly stained black with good contrast to the bone matrix in both lamellar and woven bone. In addition, the connections between the bone canaliculi and other canaliculi, osteoblasts, osteoclasts, and chondrocytes were easily detectable. We found that the bone canaliculi of woven bone were fewer in number and ran more irregularly than those of lamellar bone.We believe that this staining method for bone canaliculi in decalcified bone is superior to previously reported methods and may be useful in studies on bone pathology.