Self-aggregates of cholesterol-modified carboxymethyl konjac glucomannan conjugate: Preparation, characterization, and preliminary assessment as a carrier of etoposide
Self-aggregates of cholesterol-modified carboxymethyl konjac glucomannan conjugate: Preparation, characterization, and preliminary assessment as a carrier of etoposide
复制标题
胆固醇修饰的羧甲基魔芋葡甘聚糖缀合物的自聚集体：制备、表征和作为依托泊苷载体的初步评估
DOI：
10.1016/j.carbpol.2011.04.083
复制
发表时间：
2011-08-15
影响因子：
11.2
通讯作者：
Li, Bang-Jing
中科院分区：
文献类型：
--
作者：
Ha, Wei;Wu, Hao;Li, Bang-Jing
以羧甲基魔芋葡甘聚糖(CKGM)为亲水链段，CH为疏水部分，合成了多种含胆固醇(CH)的羧甲基魔芋葡甘聚糖(CKGM)两亲性结合物(简称CHCKGM)。用红外光谱、核磁共振氢谱和热重分析(TGA)研究了CHCKGM偶合物的结构特征。用动态激光光散射(DLS)、Zeta电位、透射电子显微镜(TEM)和荧光探针技术分析了这些自聚集体的性质。CHCKGM结合物的临界聚集浓度(CAC)较低(2.59×10~(-3)~5.89×10~(-3)mg/mL)，表明胆固醇片段是非常有效的聚集体。采用超声法将依托泊苷物理包埋到CHCKGM纳米粒中。CHCKGM纳米粒中依托泊苷的体外释放行为显示出缓释特性。此外，这些自聚集纳米颗粒表现出与pH和离子强度相关的性质，这导致它们的半径发生了相当大的变化。(C)2011爱思唯尔有限公司。保留所有权利。
Various cholesterol (CH) bearing carboxymethyl konjac glucomannan (CKGM) amphiphilic conjugates (denoted CHCKGM) were synthesized using CKGM as hydrophilic segments and CH as hydrophobic parts. Structural characteristics of these CHCKGM conjugates were investigated using FTIR, (1)H NMR and thermogravimetric analysis (TGA). The properties of these self-aggregates were analysed by dynamic laser light-scattering (DLS), zeta potential, transmission electron microscopy (TEM) and the fluorescence probe technologies. The critical aggregation concentration (cac) of CHCKGM conjugates (2.59 x 10(-3) to 5.89 x 10(-3) mg/mL) was comparatively low, suggesting that the cholesterol fragment was very effective for forming aggregates. Etoposide was physically entrapped into the CHCKGM nanoparticles by sonication method. The in vitro release behavior of etoposide from CHCKGM nanoparticles revealed a sustained release property. Furthermore, these self-aggregated nanoparticles showed pH- and ionic strength-dependent properties which caused a considerable change in their radius. (C) 2011 Elsevier Ltd. All rights reserved.