Serial backcross mapping of multiple loci associated with resistance to Leishmania major in mice
Serial backcross mapping of multiple loci associated with resistance to Leishmania major in mice
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DOI：
10.1016/s1074-7613(00)80343-x
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发表时间：
1997-05-01
期刊：
影响因子：
32.4
通讯作者：
Coffman, RL
中科院分区：
文献类型：
--
作者：
Beebe, AM;Mauze, S;Coffman, RL
近交系小鼠品系对寄生虫硕大利什曼原虫的抗性或易感性分别与Th 1或Th 2亚群的CD 4（+）T细胞应答相关。为了评估这种差异的遗传基础，将抗性B10.D2小鼠与易感BALB/c小鼠回交五代，并选择抗性。候选抗性位点的确定是通过高频率的杂合性抗性N5回交小鼠。第6、7、10、11、15和16号染色体上的基因座与抗性相关，证明了这种表型的多基因性质。所有六个基因座的存在是不必要的赋予抗性，没有一个单一的基因座是必需的。相反，这些基因座的各种组合可能能够相互作用以赋予抗性。
Resistance or susceptibility of inbred mouse strains to the parasite Leishmania major correlates with CD4(+) T cell responses of the Th1 or Th2 subsets, respectively. To evaluate the genetic basis for this difference, resistant B10.D2 mice were backcrossed onto susceptible BALB/c mice for five generations with selection for resistance. Candidate resistance loci were identified by high frequency of heterozygosity in resistant N5 backcross mice. Loci on chromosomes 6, 7, 10, 11, 15, and 16 were associated with resistance, demonstrating the multigenic nature of this phenotype. The presence of all six loci was not necessary to confer resistance and no single locus was required. Rather, a variety of combinations of these loci may be capable of interacting to confer resistance.