Actin filaments of guard cells are reorganized in response to light and abscisic acid
Actin filaments of guard cells are reorganized in response to light and abscisic acid
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DOI：
10.1104/pp.115.4.1491
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发表时间：
1997-12-01
期刊：
影响因子：
7.4
通讯作者：
Lee, YS
中科院分区：
文献类型：
--
作者：
Eun, SO;Lee, YS
我们最近发现，肌动蛋白拮抗剂的处理扰乱了鸭茅叶表皮的气孔行为，因此提出肌动蛋白的动态变化对于保卫细胞的信号响应是必要的(M.Kim，P.K.Hepler，S.O.Fun，K.S.Ha，Y.Lee[1995]Plants Physiol 109：1077-1084)。在这里，我们展示了保卫细胞的肌动蛋白细丝，通过免疫荧光显微镜观察，改变了它们的分布，以响应生理刺激。当气孔在白光照射下打开时，肌动蛋白细丝定位于保卫细胞的皮质中，排列成从气孔孔辐射出来的图案。与之形成鲜明对比的是，对于被黑暗或脱落酸封闭的气孔保卫细胞，肌动蛋白的结构特征是短片段随机定向，并沿孔位扩散标记。经脱落酸处理后，照亮的保卫细胞中的肌动蛋白阵列的放射状图案在几分钟内开始解体，并在60min时在大多数标记的保卫细胞中完全解体。与肌动蛋白细丝不同，保卫细胞的微管在所有测试条件下保持不变的组织。这些结果进一步支持肌动蛋白细丝参与保卫细胞的信号转导途径。
We recently showed that treatment with actin antagonists perturbed stomatal behavior in Commelina communis L. leaf epidermis and therefore suggested that dynamic changes in actin are necessary for signal responses in guard cells (M. Kim, P.K. Hepler, S.O. fun, K.-S. Ha, Y. Lee [1995] Plant Physiol 109: 1077-1084). Here we show that actin filaments of guard cells, visualized by immunofluorescence microscopy, change their distribution in response to physiological stimuli. When stomata were open under white-light illumination, actin filaments were localized in the cortex of guard cells, arranged in a pattern that radiates from the stomatal pore. In marked contrast, for guard cells of stomata closed by darkness or by abscisic acid, the actin organization was characterized by short fragments randomly oriented and diffusely labeled along the pore site. Upon abscisic acid treatment, the radial pattern of actin arrays in the illuminated guard cells began to disintegrate within a few minutes and was completely disintegrated in the majority of labeled guard cells by 60 min. Unlike actin filaments, microtubules of guard cells retained an unaltered organization under all conditions tested. These results further support the involvement of actin filaments in signal transduction pathways of guard cells.