Protecting murine hearts from ischaemia-reperfusion using selective inhibitors of adenosine metabolism.
Protecting murine hearts from ischaemia-reperfusion using selective inhibitors of adenosine metabolism.
复制标题
使用选择性腺苷代谢抑制剂保护小鼠心脏免受缺血再灌注。
DOI：
10.1111/j.1440-1681.2005.04168.x
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Headrick,JohnP
中科院分区：
文献类型：
--
作者：
Willems,Laura;Headrick,JohnP
1. By selectively modifying adenosine metabolism via adenosine deaminase or adenosine kinase inhibitors, it may be possible to enhance the receptor‐mediated protective actions of adenosine in a site‐ and event‐specific fashion.2. We characterized cardioprotective actions of the adenosine deaminase inhibitorerythro‐2‐(2‐hydroxy‐3‐non‐yl)adenine (EHNA) and the adenosine kinase inhibitor iodotubercidin in C57/Bl6 mouse hearts subjected to 20 min global normothermic ischaemia and 40 min reperfusion.3. Ventricular pressure development only recovered to 45 ± 2% of baseline levels (67 ± 5 mmHg) in untreated hearts, with sustained and pronounced diastolic contracture (25 ± 2 mmHg). Treatment with 20 µmol/L EHNA increased recovery of ventricular pressure (107 ± 9 mmHg), reduced postischaemic diastolic pressure (13 ± 1 mmHg) and reduced loss of lactate dehydrogenase (LDH; an indicator of necrotic damage) by 50% (9 ± 2vs19 ± 2 IU/g). Adenosine kinase inhibition with 10 µmol/L iodotubercidin also improved pressure development (to 100 ± 8 mmHg) and reduced LDH efflux (5 ± 2 IU/g).4. Protective actions were mimicked by adenosine and inhibited by adenosine receptor antagonism (50 µmol/L 8‐ρ‐sulfophenyltheophylline) and mitochondrial KATPchannel inhibition (50 µmol/L 5‐hydroxydecanoate).5. Coinfusion of the inhibitors, ‘trapping’ formed adenosine, failed to exert protection and, in some instances, was detrimental. Although substantial benefit was gained by these agents in hearts from young animals, neither inhibitor was effective in ‘aged’ hearts (18 months).6. Our data demonstrate that adenosine deaminase or kinase inhibition substantially limits injury during ischaemia–reperfusion. Protection involves adenosine receptor activation. However, cardioprotection via either enzyme inhibitor requires an alternative purine‐salvage pathway to be functional and was reduced in aged hearts known to be increasingly susceptible to ischaemic damage.
