Construction and Use of a Bottom-Up HAC Vector for Transgene Expression
Construction and Use of a Bottom-Up HAC Vector for Transgene Expression
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DOI：
10.1007/978-1-61779-099-7_7
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发表时间：
2011-01-01
期刊：
影响因子：
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通讯作者：
Suzuki, Nobutaka
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文献类型：
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作者：
Ikeno, Masashi;Suzuki, Nobutaka
最近的技术进步使得局部染色质结构的组织和动态可视化成为可能；然而，人们对染色质组织调节基因调控的整体机制知之甚少。我们设计并构建了人类人工染色体（HAG）载体，该载体可以调节转基因表达并将基因表达平台传递到许多脊椎动物细胞系中。这种使用 HAG 载体操纵转基因的技术可用于应用生物学。
Recent technological advances have enabled visualization of the organization and dynamics of local chromatin structures; however, the global mechanisms by which chromatin organization modulates gene regulation are poorly understood. We designed and constructed a human artificial chromosome (HAG) vector that allows regulation of transgene expression and delivery of a gene expression platform into many vertebrate cell lines. This technology for manipulating a transgene using a HAG vector could be used in applied biology.