Bioluminescence: a versatile technique for imaging cellular and molecular features.
Bioluminescence: a versatile technique for imaging cellular and molecular features.
复制标题
DOI：
10.1039/c3md00288h
复制
发表时间：
2014-03-01
期刊：
影响因子：
--
通讯作者：
Prescher JA
中科院分区：
文献类型：
--
作者：
Paley MA;Prescher JA
生物发光是一种普遍存在的成像方式，用于可视化生物体内的过程。这项技术利用可见光，并且很容易与大多数细胞和组织类型接触，使其成为临床前研究的通用技术。在这里，我们回顾了基本的生物发光成像原理，以及与药物化学社区相关的技术应用。这些包括无创细胞跟踪实验、蛋白质功能分析和小分子代谢物可视化方法。在每个部分中，我们还讨论了生物发光工具如何揭示实验疗法和辅助药物发现的见解。最后，我们强调了新的生物发光工具的发展，这将使更敏感和多组分成像实验，从而扩大我们对生命系统的更广泛的理解。
Bioluminescence is a ubiquitous imaging modality for visualizing biological processes in vivo. This technique employs visible light and interfaces readily with most cell and tissue types, making it a versatile technology for preclinical studies. Here we review basic bioluminescence imaging principles, along with applications of the technology that are relevant to the medicinal chemistry community. These include noninvasive cell tracking experiments, analyses of protein function, and methods to visualize small molecule metabolites. In each section, we also discuss how bioluminescent tools have revealed insights into experimental therapies and aided drug discovery. Last, we highlight the development of new bioluminescent tools that will enable more sensitive and multi-component imaging experiments and, thus, expand our broader understanding of living systems.