Identification and Characterization of a Novel Hepta-Segmented dsRNA Virus From the Phytopathogenic Fungus Colletotrichum fructicola.
Identification and Characterization of a Novel Hepta-Segmented dsRNA Virus From the Phytopathogenic Fungus Colletotrichum fructicola.
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植物病原真菌果炭疽菌 (Colletotrichum fructicola) 中新型七段 dsRNA 病毒的鉴定和表征
DOI：
10.3389/fmicb.2018.00754
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发表时间：
2018
影响因子：
5.2
通讯作者：
Wang G
中科院分区：
文献类型：
--
作者：
Zhai L;Zhang M;Hong N;Xiao F;Fu M;Xiang J;Wang G
从植物病原真菌Colletotrichum fructicola菌株FJ-4中分离到一种新的七节段双链RNA病毒，命名为Colletotrichum fructicola chrysovirus 1（CfCV 1）。dsRNA 1 -7的全长cDNA分别为3620、2801、2687、2437、1750、1536和1211 bp。7个dsRNA的5′-和3′-非翻译区具有高度相似的内部序列，并含有保守的序列延伸，表明它们具有共同的病毒起源。预测7个dsRNA的5′-和3′-UTR折叠成稳定的茎环结构。CfCV 1含有直径为35 nm的球形病毒体，由7个片段组成。CfCV 1的最大dsRNA编码RNA依赖性RNA聚合酶（RdRp），第二dsRNA编码病毒衣壳蛋白（CP）。dsRNA 5编码一个C2 H2型锌指蛋白，含有一个富R区和一个富G区。最小的dsRNA是卫星状RNA。由dsRNA 3、dsRNA 4、dsRNA 6编码的其他蛋白质的功能尚不清楚。系统发育分析表明，CfCV 1与Botryosphaeria dothidea chrysovirus 1（BdCV 1）和Penicillium janczewskii chrysovirus 2（PjCV 2）有亲缘关系，后者是金病毒科中一个独立的簇II组的一个簇。重要的是，CfCV 1的所有七个片段都以全有或全无的方式成功传播给其他无病毒株。CfCV 1对C.但可以赋予真菌宿主低毒力。据我们所知，这是第一次在C.果生菌
A novel hepta-segmented double-stranded RNA (dsRNA) virus was isolated and characterized from the strain FJ-4 of the phytopathogenic fungus Colletotrichum fructicola, and was named Colletotrichum fructicola chrysovirus 1 (CfCV1). The full-length cDNAs of dsRNA1–7 were 3620, 2801, 2687, 2437, 1750, 1536, and 1211 bp, respectively. The 5′- and 3′-untranslated regions of the seven dsRNAs share highly similar internal sequence and contain conserved sequence stretches, indicating that they have a common virus origin. The 5′-and 3′-UTRs of the seven dsRNAs were predicted to fold into stable stem-loop structures. CfCV1 contains spherical virions that are 35 nm in diameter consisting of seven segments. The largest dsRNA of CfCV1 encodes an RNA-dependent RNA polymerase (RdRp), and the second dsRNA encodes a viral capsid protein (CP). The dsRNA5 encodes a C2H2-type zinc finger protein containing an R-rich region and a G-rich region. The smallest dsRNA is a satellite-like RNA. The functions of the other proteins encoded by dsRNA3, dsRNA4, dsRNA6 are unknown. Phylogenetic analysis, based on RdRp and CP, indicated that CfCV1 is phylogenetically related to Botryosphaeria dothidea chrysovirus 1 (BdCV1), and Penicillium janczewskii chrysovirus 2 (PjCV2), a cluster of an independent cluster II group in the family Chrysoviridae. Importantly, all the seven segments of CfCV1 were transmitted successfully to other virus-free strains with an all-or-none fashion. CfCV1 exerts minor influence on the growth of C. fructicola but can confer hypovirulence to the fungal host. To our knowledge, this is the first report of a hepta-segmented tentative chrysovirus in C. fructicola.