Serotyping of Toxoplasma gondii:: striking homogeneous pattern between symptomatic and asymptomatic infections within Europe and South America
Serotyping of Toxoplasma gondii:: striking homogeneous pattern between symptomatic and asymptomatic infections within Europe and South America
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DOI：
10.1016/j.micinf.2008.04.001
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发表时间：
2008-06-01
影响因子：
5.8
通讯作者：
Cesbron-Delauw, Marie-France
中科院分区：
文献类型：
--
作者：
Morisset, Stephane;Peyron, Francois;Cesbron-Delauw, Marie-France
欧洲和北美的弓形虫野外分离株被分为三个克隆谱系，它们在小鼠中表现出不同的毒力。该寄生虫的遗传结构是否与人类的临床表达有关尚未得到证实。我们开发了一种酶联免疫吸附试验，它使用谱系特异性，多态性多肽来自致密颗粒抗原，GRA 5和GRA 6。我们的目标是比较在欧洲和南美患者中观察到的无症状与有症状（人类免疫缺陷病毒（HIV）阳性患者中的眼部疾病和严重感染）感染的血清型模式。与疾病的临床表现无关，血清型根据地理来源而不同，血清型11在欧洲均匀分布，血清型I和III在南美洲均匀分布。我们的结论是，GRA 5-GRA 6血清分型是一个有趣的工具，研究血清型流行的人群，但它不是一个准确的标记弓形虫感染的致病性在人类。(C)2008年，Elsevier Masson SAS。All rights reserved.
Field isolates of Toxoplasma gondii in Europe and North America have been grouped into three clonal lineages that display different virulence in mice. Whether the genetic structure of the parasite is related to clinical expression in humans has not yet been demonstrated. We developed an enzyme-linked immunosorbent assay which uses lineage-specific, polymorphic polypeptides derived from the dense granule antigens, GRA5 and GRA6. Our goal was to compare serotypical patterns observed in asymptomatic versus symptomatic (ocular disease and severe infection in human immunodeficiency virus (HIV)-positive patients) infections among patients from Europe and South America. Independent of the clinical presentation of the disease, serotypes differed according to geographical origin, with a homogeneous distribution of serotype 11 in Europe and of serotypes I and III in South America. We conclude that GRA5-GRA6 serotyping is an interesting tool to study serotype prevalence in populations but it is not an accurate marker of pathogenicity of Toxoplasma infection in humans. (C) 2008 Elsevier Masson SAS. All rights reserved.