GATA3 is downregulated in osteosarcoma and facilitates EMT as well as migration through regulation of slug.
GATA3 is downregulated in osteosarcoma and facilitates EMT as well as migration through regulation of slug.
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DOI：
10.2147/ott.s176534
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发表时间：
2018
影响因子：
4
通讯作者：
Ma X
中科院分区：
文献类型：
--
作者：
Ma L;Xue W;Ma X
GATA3作为一种肿瘤抑制因子，已在多种类型的癌症中被观察到，但GATA3在骨肉瘤（OS）中的作用和机制尚不清楚。采用定量反转录PCR和Western blotting检测OS细胞和组织中GATA3的表达。采用CCK-8法、菌落形成法、伤口愈合法和transwell法检测GATA3对细胞增殖、迁移和侵袭的影响。通过ChIP和qChIP以及荧光素酶检测GATA3是否通过转录调控鼻涕虫的表达。在OS细胞和组织中GATA3表达下调。GATA3的表达与肿瘤大小及转移密切相关。GATA3显著抑制OS细胞的增殖、迁移和侵袭。EMT相关转录因子slug被GATA3转录抑制，从而调控了OS中的EMT。GATA3在OS中作为肿瘤抑制因子，通过调控slug抑制OS的进展和转移。
GATA3 functions as a tumor suppressor and has been observed in multiple types of cancer, but the effects and mechanisms of GATA3 in osteosarcoma (OS) are not yet known. The GATA3 expression in OS cells and tissues were detected using quantitative reverse-transcription PCR and Western blotting assay. CCK-8 assay, colony formation assay, wound healing assay as well as transwell assay, were performed to determine the effects of GATA3 on cell proliferation, migration and invasion. ChIP and qChIP as well as luciferase assay were performed whether GATA3 transcriptionally regulated slug expression. GATA3 was downregulated in OS cells and tissues. The GATA3 expression was closely associated with tumor size as well as metastasis. GATA3 significantly suppressed OS cells proliferation, migration and invasion. EMT-associated transcript factor, slug, was transcriptionally inhibited by GATA3, thereby regulation of EMT in OS. GATA3 serves as a tumor suppressor in OS and suppresses the progression and metastasis of OS through regulation of slug.