Comparative analysis of semaphorin 3A in soleus and EDL muscle satellite ceels in vitro toward understanding its role in modulating
Comparative analysis of semaphorin 3A in soleus and EDL muscle satellite ceels in vitro toward understanding its role in modulating
复制标题
体外比较分析比目鱼肌和 EDL 肌肉卫星细胞中的信号蛋白 3A，以了解其调节作用
DOI：
10.1016/j.biocel.2012.10.003
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Suzuki T et aj.
中科院分区：
文献类型：
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作者：
Singh S;Rajendran R;Kuroda K;Isogai E;Kuristic-Demonakos M;Demonakos CD;Suzuki T et aj.
常驻肌源性干细胞，卫星细胞，上调分泌的多功能调节剂，脑信号蛋白3A（Sema 3A），专门在早期分化阶段，以响应肌肉挤压损伤和治疗与肝细胞生长因子（HGF）或碱性成纤维细胞生长因子（FGF 2）。在这里，我们增加的证据表明，Sema 3A的表达和分泌诱导的生长因子是显着较高的成年大鼠比目鱼肌比从快速收缩伸趾长肌（EDL）肌肉的原代培养物。较高的Sema 3A反应，揭示了定量PCR和Western印迹的细胞裂解物和条件培养基，可能占较高的肌细胞生成素表达的比目鱼肌卫星细胞在分化早期，因为添加重组Sema 3A刺激肌细胞生成素的表达在文化。这些实验还表明，丛蛋白A2的mRNA表达，它与神经纤毛蛋白，构成Sema 3A复合受体，是高于卫星细胞从比目鱼肌比EDL，丛蛋白A1和A3和神经纤毛蛋白-1和2水平没有差异。因此，这些比较研究突出了一个可能的Sema 3A-丛蛋白A2-肌细胞生成素信号轴，可以确保促进比目鱼肌卫星细胞的早期分化。
Resident myogenic stem cells, satellite cells, up-regulate a secreted multi-functional modulator, semaphorin 3A (Sema3A), exclusively at the early-differentiation phase in response to muscle-crush injury and treatment with hepatocyte growth factor (HGF) or basic fibroblast growth factor (FGF2). Here, we add evidence that the Sema3A expression and secretion induced by the growth factors is significantly higher in primary cultures from adult rat soleus than from the fast-twitch extensor digitorum longus (EDL) muscle. The higher Sema3A response, revealed by quantitative PCR and Western blotting of cell lysates and conditioned media, may account for the higher myogenin expression of soleus muscle satellite cells early in differentiation since addition of recombinant Sema3A stimulates myogenin expression in cultures. These experiments also showed that mRNA expression of plexin A2, which together with neuropilins, constitutes Sema3A composite-receptors, was higher in satellite cells from soleus than EDL with no difference in plexin A1 and A3 and neuropilin-1 and 2 levels. These comparative studies, therefore, highlight a possible Sema3A-plexin A2-myogenin signaling axis that may ensure promoting early differentiation by soleus muscle satellite cells.