Hepatitis E virus load in swine organs and tissues at slaughterhouse determined by real-time RT-PCR
Hepatitis E virus load in swine organs and tissues at slaughterhouse determined by real-time RT-PCR
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DOI：
10.1016/j.ijfoodmicro.2010.02.016
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发表时间：
2010-05-15
影响因子：
5.4
通讯作者：
Houde, Alain
中科院分区：
文献类型：
--
作者：
Leblanc, Danielle;Poitras, Elyse;Houde, Alain
尽管戊型肝炎病毒（HEV）在北美并不常见，但在一些工业化国家中，在没有前往戊型肝炎流行国家旅行史的患者中发现了戊型肝炎病毒。它在全世界的猪群中普遍存在。猪戊型肝炎病毒在非人类灵长类动物中的人畜共患传播已在实验中实现，并且在摄入受污染的生肉或未煮熟的肉后传播戊型肝炎病毒已得到充分记录。在加拿大，到目前为止还没有REV暴发的记录，但在猪源性的血清和粪便样本中发现了HEV基因3型毒株。本研究的目的是测定43头屠宰猪的肝脏、腰部、膀胱、肝淋巴结、胆汁、扁桃体、血浆和粪便样本中的HEV病毒载量。使用猫杯状病毒（FCV）作为样品过程对照，验证RNA提取过程，确认不存在样品抑制剂，并作为扩增对照。利用FCV/HEV多重TaqMan RT-qPCR系统，43只实验动物中有14只检测到REV RNA。感染动物的淋巴结（11/43）、膀胱（10/43）、肝脏（9/43）、胆汁（8/43）、粪便（6/43）、扁桃体（3/43）、血浆（1/43）均检测到REV。未观察到hev阳性的腰部标本。在阳性肝脏和胆汁样本中，估计病毒载量为10(3)至10(7)拷贝/g。皇冠版权所有(C) 2010由Elsevier B.V.出版。
Although uncommon in North America, Hepatitis E virus (HEV) has been identified in some industrialized countries in patients without a history of travel to HEV-endemic countries. Its presence is ubiquitous worldwide in swine populations. Zoonotic transmission of swine HEV to non human primates has been achieved experimentally and transmission of HEV after ingestion of contaminated raw or undercooked meat is well documented. In Canada, so far, no REV outbreak has been documented but HEV genotype 3 strains have been identified in sera and faecal samples of swine origin. The objective of the present study was to determine the viral load of HEV in liver, loin, bladder, hepatic lymph node, bile, tonsil, plasma and faeces samples of 43 pigs at slaughter. Feline calicivirus (FCV) was used as sample process control to validate the RNA extraction process, as a confirmation of the absence of sample inhibitors and as an amplification control. Using FCV/HEV multiplex TaqMan RT-qPCR system, REV RNA was detected in 14 out of the 43 animals tested. REV was detected in lymph nodes (11/43), bladder (10/43), liver (9/43), bile (8/43), faeces (6/43), tonsils (3/43), plasma (1/43) samples from infected animals. No HEV-positive loin samples were observed. Viral loads of 10(3) to 10(7) copies/g were estimated in positive liver and bile samples. Crown Copyright (C) 2010 Published by Elsevier B.V. All rights reserved.