Impact of fetal exposure to mycotoxins on longissimus muscle fiber hypertrophy and miRNA profile.
Impact of fetal exposure to mycotoxins on longissimus muscle fiber hypertrophy and miRNA profile.
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DOI：
10.1186/s12864-022-08794-0
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发表时间：
2022-08-16
期刊：
影响因子：
4.4
通讯作者：
Duckett, S. K.
中科院分区：
文献类型：
--
作者：
Greene, M. A.;Udoka, A. N. S.;Powell, R. R.;Noorai, R. E.;Bruce, T.;Duckett, S. K.
最长肌样本是从妊娠中期（MID；E + /E-）或妊娠晚期（LATE；E-/E +）期间在子宫内暴露于霉菌毒素的羔羊身上采集的[E-，不含内生菌且不含麦角生物碱的高羊茅种子（阴性对照）或E + ，内生菌感染的高羊茅种子，含有麦角生物碱]在两个发育阶段（FETAL，妊娠d133）或（MAT，接近成熟，250天龄；n = 3/处理/发育阶段）收获。检查肌肉样本以确定子宫内霉菌毒素暴露对骨骼肌纤维肥大的影响以及 FETAL 和 MAT 的 miRNA 谱。在 MAT 中，与 E-/E + 羔羊相比，E + /E- 羔羊的最长肌重量更大 (P < 0.05)；然而，羊茅处理之间的胎儿最长肌重量没有差异（P > 0.10）。在 MAT 上，E + /E- 的 I 型纤维横截面积大于 (P < 0.10) 比 E-/E + 在 FETAL 上的羊茅处理之间没有差异 (P > 0.10)。与 E-/E + 相比，E + /E- 中 MAT 的 II 型纤维面积更大 (P < 0.05)，但 FETAL 的羊茅处理之间没有差异 (P < 0.05)。从 FETAL 到 MAT，I 型和 II 型最长肌纤维横截面面积分别增加了 6.86 倍和 10.83 倍（P < 0.05）。与 FETAL 相比，MAT 中 II 型肌纤维与 I 型肌纤维的比例较低 (P = 0.04)。 FETAL 和 MAT 之间有 120 个差异表达的 miRNA（q< 0.05）。母体羊茅处理没有改变最长肌中 miRNA 的表达（q > 0.05）。 miR-133、-29a、-22-3p 和 -410-3p 被鉴定为高度显着，log2 倍数变化≥4。体外卫星细胞培养显示，选定的 miRNA（miR-22-3p、29a、27a 和 133a）在增殖和分化过程中受到差异性调节，表明 miRNA 在肌肉肥大中的作用。接触霉菌毒素不会改变纤维类型，但会对出生后肌肉肥大和横截面积产生长期影响。最长肌的 miRNA 谱未因 FETAL 或 MAT 时母体霉菌毒素暴露而改变。发育年龄改变了与肌肉生长相关的已知基因的 miRNA 转录组和 mRNA 表达。这些结果表明，母体在妊娠晚期接触 E + 羊茅种子可以改变绵羊产后肌肉肥大；然而，这些变化不受最长肌 miRNA 转录组的调节。在线版本包含可在 10.1186/s12864-022-08794-0 获取的补充材料。
Longissimus muscle samples were collected from lambs exposed in utero to mycotoxins [E-, endophyte-free tall fescue seed without ergot alkaloids (negative control) or E + , endophyte-infected tall fescue seed containing ergot alkaloids] during mid-gestation (MID; E + /E-) or late-gestation (LATE; E-/E +) harvested at two developmental stages (FETAL, gestational d133) or (MAT, near maturity, 250 d of age; n = 3/treatment/developmental stage). Muscle samples were examined to determine the impact of in utero mycotoxin exposure on skeletal muscle fiber hypertrophy and the miRNA profile at FETAL and MAT. Longissimus weight was greater (P < 0.05) in E + /E- lambs compared to E-/E + lambs at MAT; however, FETAL longissimus weight did not differ (P > 0.10) between fescue treatments. Type I fiber cross sectional area was larger (P < 0.10) for E + /E- than E-/E + at MAT but did not differ (P > 0.10) between fescue treatments at FETAL. Type II fiber area was larger (P < 0.05) at MAT in E + /E- compared to E-/E + but did not differ (P < 0.05) between fescue treatments at FETAL. Cross-sectional Type I and Type II longissimus muscle fiber area increased (P < 0.05) from FETAL to MAT by 6.86-fold and 10.83-fold, respectively. The ratio of Type II:Type I muscle fibers was lower (P = 0.04) at MAT compared to FETAL. There were 120 miRNA differentially expressed (q < 0.05) between FETAL and MAT. Maternal fescue treatment did not alter (q > 0.05) expression of miRNAs in the longissimus muscle. miR-133, -29a, -22-3p, and -410-3p were identified as highly significant with a log2 fold change > 4. In vitro satellite cell cultures showed that selected miRNAs (miR-22-3p, 29a, 27a, and 133a) are differentially regulated during proliferation and differentiation indicating a role of miRNA in muscle hypertrophy. Exposure to mycotoxins did not alter fiber type but had long-term impacts on postnatal muscle hypertrophy and cross-sectional area. The miRNA profile of the longissimus was not altered by Maternal mycotoxin exposure at FETAL or MAT. Developmental age altered the miRNA transcriptome and mRNA expression of known genes related to muscle growth. These results indicate that Maternal exposure to E + fescue seed during LATE gestation can alter postnatal muscle hypertrophy in sheep; however, these changes are not regulated by the miRNA transcriptome of the longissimus muscle. The online version contains supplementary material available at 10.1186/s12864-022-08794-0.
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影响因子：
2.7
作者：
Day, Kenneth;Shefer, Gabi;Yablonka-Reuveni, Zipora
通讯作者：
Yablonka-Reuveni, Zipora
影响因子：
4.9
作者：
Farina, Nicholas H.;Hausburg, Melissa;Olwin, Bradley B.
通讯作者：
Olwin, Bradley B.
影响因子：
1.9
作者：
Greenwood, PL;Slepetis, RM;Bell, AW
通讯作者：
Bell, AW
影响因子：
3.3
作者：
ABERLE, ED
通讯作者：
ABERLE, ED
DOI：
10.3390/ani10101859
发表时间：
2020-10-12
期刊：
Animals : an open access journal from MDPI
影响因子：
--
作者：
Greene MA;Britt JL;Bertrand JK;Klotz JL;Bridges W Jr;Andrae JG;Duckett SK
通讯作者：
Duckett SK