Synthesis of nanophase hydroxyapatite by a Serratia sp. from waste-water containing inorganic phosphate
Synthesis of nanophase hydroxyapatite by a Serratia sp. from waste-water containing inorganic phosphate
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沙雷菌属纳米相羟基磷灰石的合成。
DOI：
10.1007/s10529-004-3744-4
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发表时间：
2004
影响因子：
2.7
通讯作者：
P. Marquis
中科院分区：
文献类型：
--
作者：
P. Yong;L. Macaskie;R. Sammons;P. Marquis
以CaCl 2和无机磷酸盐（Pi）为代价，在细菌表面合成纳米羟基磷灰石（HA）。初始成核后，钙以磷酸钙形式沉淀在细胞上和周围，而挑战溶液中的无机磷酸盐则为代价，无细胞对照中无沉淀。HA也使用从含磷酸盐的废水中清除的无机磷酸盐离子进行生物制造。加入Ca ~（2+）后，废水中磷酸盐浓度由0.27（~ 125 ppm）降至~ 10.02m（~ 122 ppm）。通过扫描电子显微镜（SEM）定位由细胞制造的磷酸钙晶体，并通过X射线粉末衍射鉴定为HA，平均晶体尺寸计算为约25 nm。可能的应用bioHA作为一种生物材料和一步'废物转化为产品'的影响进行了讨论。
Synthesis of nanophase hydroxyapatite (HA) on a bacterial surface was achieved at the expense of CaCl2 and inorganic phosphate (Pi). After initial nucleation, calcium was precipitated on and around the cells as calcium phosphate at the expense of inorganic phosphate in the challenge solution, with no precipitation in cell-free controls. HA was also biomanufactured using inorganic phosphate ions scavenged from a phosphate-containing waste-water. With additional Ca2+, the concentration of phosphate was decreased from 0.27 (∼25 ppm) to ∼0.02m (∼2 ppm) in the waste-water. Crystals of calcium phosphate manufactured by the cells were located by scanning electron microscopy (SEM) and identified as HA by X-ray powder diffraction, with an average crystal size calculated as ∼25 nm. Possible application of bioHA as a biomaterial and implications for one-step `waste-into product' are discussed.