Quantification of human tissue kallikreins in the stratum corneum: Dependence on age and gender
Quantification of human tissue kallikreins in the stratum corneum: Dependence on age and gender
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DOI：
10.1111/j.0022-202x.2005.23933.x
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发表时间：
2005-12-01
影响因子：
6.5
通讯作者：
Diamandis, EP
中科院分区：
文献类型：
--
作者：
Komatsu, N;Saijoh, K;Diamandis, EP
人组织嗜碱性蛋白酶是一个由15个胰蛋白酶或凝乳胰蛋白酶样分泌丝氨酸蛋白酶（hK1-hK15）组成的家族。在角质层（SC）、颗粒层和皮肤附属物中发现了hK5、hK6、hK7、hK8和hK13。据报道，hK5和hK7降解桥粒/角质层桥粒，表明钾样蛋白与脱屑有关。我们报告了用ELISA定量SC中hK5、hK6、hK7、hK8、hK10、hK11、hK13和hK14的含量及其在不同年龄组间的变化。测定SC总胰蛋白酶和凝乳胰蛋白酶样活性。hK7、hK8和hK11 （ng / mg干重）含量较高，在6 ~ 14之间变化，hK5（2.0 ~ 4.0）含量居中，hK10（0.65 ~ 1.0）、hK14（0.1 ~ 0.3）、hK6（0.1 ~ 0.3）和hK13（0.02 ~ 0.1）含量较低。hK6和hK14在20 ~ 59岁的女性中显著降低，hK5、hK7、hK10、hK11和hK14在不同年龄组间差异不显著。hK8在极端年龄时最低（30-39岁时最高），hK6在30- 30岁时较低，hK13在10 - 20岁时较低。总体胰蛋白酶样活性在不同年龄组之间没有差异，但在< 11岁的受试者中较高。总体凝乳胰蛋白酶样活性与年龄无关。总之，我们在SC中发现了多个钾化酶，并表明这些酶可能通过酶级联途径负责脱屑。
Human tissue kallikreins are a family of 15 trypsin or chymotrypsin-like secreted serine proteases (hK1-hK15). hK5, hK6, hK7, hK8, and hK13 have been identified in the stratum corneum (SC), stratum granulosum, and skin appendages. It has been reported that hK5 and hK7 degrade desmosomes/corneodesmosomes, suggesting that kallikreins are responsible for desquamation. We report the quantification of hK5, hK6, hK7, hK8, hK10, hK11, hK13, and hK14 in the SC by ELISA and their variation among age groups. The total SC trypsin and chymotrypsin-like activities were also measured. The amount of hK7, hK8, and hK11 (ng per mg dry weight) were high, and varied from 6 to 14, hK5 (2.0-4.0) was present at intermediate levels,and hK10 (0.65-1.0), hK14 (0.1-0.3), hK6 (0.1-0.3),and hK13 (0.02-0.1) were present at lower levels. hK6 and hK14 were significantly lower in females between 20 and 59 y. hK5, hK7, hK10, hK11, and hK14 were not significantly different across the age groups. hK8 was lowest at extremes of age (highest at 30-39 y), hK6 was lower at > 30 y, and hK13 was lower at > 20 y. Overall trypsin-like activity did not differ across age groups but was higher in subjects < 11 y. Overall chymotrypsin-like activity was not related to age. In conclusion, we found multiple kallikreins in the SC and suggest that these enzymes may be responsible for desquamation through an enzymatic cascade pathway.