Insulin Stimulation of Protein Synthesis and mTOR Signaling in Chick Myotube Cultures
Insulin Stimulation of Protein Synthesis and mTOR Signaling in Chick Myotube Cultures
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DOI：
10.2141/jpsa.0190082
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发表时间：
2020-07-01
影响因子：
1.5
通讯作者：
Ohtsuka, Akira
中科院分区：
文献类型：
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作者：
Nakashima, Kazuki;Ishida, Aiko;Ohtsuka, Akira
胰岛素刺激骨骼肌中的蛋白质合成。蛋白质合成由骨骼肌中雷帕霉素 (mTOR) 信号传导的机械靶点控制。本研究旨在研究胰岛素对鸡肌管培养物中蛋白质合成和 mTOR 信号传导的影响。将鸡肌管与胰岛素(1μg/ml)一起孵育1小时。使用平移表面传感方法测量的蛋白质合成通过胰岛素显着增加。胰岛素也显着增加 AKT（Thr308 和 Ser473）、p70 核糖体 S6 激酶 1（S6K1、Thr389）、S6 核糖体蛋白（Ser235/236）和真核翻译起始因子 4E 结合蛋白 1（4E-BP1、Thr37/46）的磷酸化。这些结果表明，在鸡肌管培养物中，胰岛素通过 mTOR 信号传导（AKT、S6K1、S6 核糖体蛋白和 4E-BP1 的磷酸化）刺激蛋白质合成。
Insulin stimulates protein synthesis in skeletal muscles. Protein synthesis is controlled by the mechanistic target of rapamycin (mTOR) signaling in skeletal muscles. This study was conducted to investigate the effect of insulin on protein synthesis and mTOR signaling in chick myotube cultures. Chick myotubes were incubated with insulin (1 mu g/ml) for 1 h. Protein synthesis, measured using the surface sensing of translation method, was significantly increased by insulin. The phosphorylation of AKT (Thr308 and Ser473), p70 ribosomal S6 kinase 1 (S6K1, Thr389), S6 ribosomal protein (Ser235/236), and eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1, Thr37/46) was also significantly increased by insulin. These results suggest that insulin stimulates protein synthesis via mTOR signaling (phosphorylation of AKT, S6K1, S6 ribosomal protein, and 4E-BP1) in chick myotube cultures.