Genome-wide identification of apple PPI genes and a functional analysis of the response of MxPPI1 to Fe deficiency stress.
Genome-wide identification of apple PPI genes and a functional analysis of the response of MxPPI1 to Fe deficiency stress.
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DOI：
10.1016/j.plaphy.2022.08.017
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发表时间：
2022-08
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影响因子：
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通讯作者：
Min Gao;Qiran Sun;L. Zhai;Danrui Zhao;Jiahong Lv;Zhenhai Han;Ting Wu;Xinzhong Zhang;Xuefeng Xu;Yi Wang
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文献类型：
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作者：
Min Gao;Qiran Sun;L. Zhai;Danrui Zhao;Jiahong Lv;Zhenhai Han;Ting Wu;Xinzhong Zhang;Xuefeng Xu;Yi Wang
缺铁影响植物的生长发育。植物体内的质子泵互作子（PPI）对多种非生物胁迫有响应，但在缺铁胁迫下其功能尚未得到很好的表征。本研究对苹果PPI基因家族进行了系统的鉴定和分析。发现了3个PPI候选基因，它们含有318-1349个氨基酸和3-7个内含子。在缺铁胁迫下，分析了苹果砧木小金海棠根中所有PPI基因的表达。缺铁处理明显诱导M.我们首先从M.并对小金刺进行了亚细胞定位，结果表明，小金刺定位于烟草的细胞膜和细胞核中。我们发现，在苹果愈伤组织缺铁胁迫下，MxPPI 1蛋白的表达水平显着增加。与野生型相比，过量表达MxPPI 1的苹果愈伤组织铁螯合还原酶和H+-ATP酶活性、H+分泌、MxHA 2基因表达和总铁含量均有所提高。我们进一步发现，MxPPI 1与MxHA 2相互作用，使用双分子荧光互补和荧光素酶互补试验。总之，我们证明了MxPPI 1与MxHA 2相互作用，增强H+-ATP酶的活性，从而调节M对Fe的吸收。小金藻
Iron (Fe) deficiency affects plant growth and development. The proton pump interactor (PPI) in plants responds to multiple abiotic stresses, although it has not been well characterized under Fe deficiency stress. In this study, we systematically identified and analyzed thePPIgene family in apple. ThreePPIcandidate genes were found, and they contained 318–1349 amino acids and 3–7 introns. Under Fe deficiency stress, we analyzed the expression of all thePPIgenes in roots of apple rootstock Malusxiaojinensis. Expression of the gene MD11G1247800, designatedPPI1, is obviously induced by Fe deficiency treatment inM. xiaojinensis.We first clonedMxPPI1fromM. xiaojinensisand determined its subcellular localization, which indicated that it is localized in the cell membrane and nucleus in tobacco. We found that the level of expression of the MxPPI1 protein increased significantly under Fe deficiency stress in apple calli. Moreover, overexpressingMxPPI1in apple calli enhanced the activities of ferric chelate reductase and H+-ATPase, H+secretion,MxHA2gene expression and total Fe content when compared with the wild type calli. We further found that MxPPI1 interacted with MxHA2 using bimolecular fluorescence complementation and luciferase complementation assays. Overall, we demonstrated that MxPPI1 interacts with MxHA2 to enhance the activity of H+-ATPase to regulate Fe absorption inM. xiaojinensis.