The expression of scavenger receptor class B, type I (SR-BI) and caveolin-1 in parenchymal and nonparenchymal liver cells
The expression of scavenger receptor class B, type I (SR-BI) and caveolin-1 in parenchymal and nonparenchymal liver cells
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DOI：
10.1007/s00441-001-0476-9
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发表时间：
2002-02-01
影响因子：
3.6
通讯作者：
Berg, T
中科院分区：
文献类型：
--
作者：
Malerod, L;Juvet, LK;Berg, T
肝脏是胆固醇合成和代谢的主要部位，也是消除血液中胆固醇的唯一实质性途径。据报道，清道夫受体B类，I型（SR-BI）负责介导高密度脂蛋白胆固醇酯（HDL-CE）在肝实质细胞（PC）中的选择性摄取。我们分析了SR-BI在离体大鼠肝细胞中的表达，发现该受体在肝PC中的mRNA和蛋白水平均有高表达。我们还发现SR-BI在肝内皮细胞（LEC）和库普弗细胞（KC）中表达。SR-BI以前没有报道在LEC中存在。cd36mrna在三种肝细胞中均有表达。由于caveolin-1似乎与SR-BI和CD36在几种细胞系的小泡中共定位，因此研究了caveolin-1在肝细胞中的分布和表达。在PC中未检测到小窝蛋白-1，但在LEC和KC中均有发现，这表明小窝蛋白-1可能在胆固醇外排中比在肝脏中胆固醇的选择性摄取中更重要。
The liver is the major site of cholesterol synthesis and metabolism, and the only substantive route for eliminating blood cholesterol. Scavenger receptor class B, type I (SR-BI) has been reported to be responsible for mediating the selective uptake of high-density lipoprotein cholesteryl esters (HDL-CE) in liver parenchymal cells (PC). We analysed the expression of SR-BI in isolated rat liver cells, and found the receptor to be highly expressed in liver PC at both the mRNA and protein levels. We also found SR-BI to be expressed in liver endothelial cells (LEC) and Kupffer cells (KC). SR-BI has not previously been reported to be present in LEC. CD36 mRNA was expressed in all three liver cell types. Since caveolin-1 appears to colocalize with SR-BI and CD36 in caveolae of several cell lines, the distribution and expression of caveolin-1 in the liver cells were investigated. Caveolin-1 was not detected in PC but was found in both LEC and KC. This led to the suggestion that caveolin-1 may be more important in the efflux of cholesterol than in the selective uptake of cholesterol in the liver.