A polygalacturonase inhibitory protein gene (BcMF19) expressed during pollen development in Chinese cabbage-pak-choi
A polygalacturonase inhibitory protein gene (BcMF19) expressed during pollen development in Chinese cabbage-pak-choi
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DOI：
10.1007/s11033-010-0139-6
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发表时间：
2011-01-01
影响因子：
2.8
通讯作者：
Cao, Jiashu
中科院分区：
文献类型：
--
作者：
Huang, Li;Liu, Ying;Cao, Jiashu
参与花粉发育的多聚半乳糖醛酸酶抑制蛋白(PGIP)基因的水平尚不清楚。鉴定在花粉中表达的不同的PGIP基因对于了解该基因家族成员之间的功能异同以及花粉发育的潜在机制是必要的。在大白菜(Brassica campestris L.SSP)可育花蕾中发现了一个编码PGIP的基因BcMF19，该基因在可育花蕾中表达上调。中国)核不育AB系(Bajh97-01A/B)。BcMF19的氨基酸序列具有PGIPs的基本特征，包括一个N-末端信号肽、几个潜在的N-糖基化位点、N-末端和C-末端两侧的两个二硫键以及10个富亮氨酸重复序列(LRR)。实时荧光定量RT-PCR验证了BcMF19在可育花蕾中的表达高于不育花蕾。原位杂交结果表明，在花药发育过程中，BcMF19仅在绒被细胞和小孢子中表达。这些结果表明，BcMF19是一个新的PGIP基因，可能与花粉或绒毡层发育有关。
The level of polygalacturonase inhibitory protein (PGIP) genes involved in pollen development remains unclear. Characterization of the different PGIP genes that are expressed in pollen is necessary in understanding the similarities and differences of functions between the members of this gene family, as well as the underlying mechanism of pollen development. A gene-encoding putative PGIP, BcMF19 was successfully cloned on a cDNA-amplified fragment length polymorphism fragment after it was found to be up-regulated in the fertile flower buds of Chinese cabbage-pak-choi (Brassica campestris L. ssp. chinensis Makino) genic male sterile AB line (Bajh97-01A/B). The amino acid sequence of BcMF19 possessed the basic feature of PGIPs, containing an N-terminal signal peptide, several potential N-glycosylation sites, two disulfide bridges flanking both the N- and C-terminal regions, and 10 leucine-rich repeat (LRR) consensus sequences. Real-time RT-PCR verified the higher expression of BcMF19 in the fertile flower buds compared to the sterile flower buds. In situ hybridization showed that BcMF19 was exclusively expressed in the tapetal cells and microspores during anther development. These results indicate that BcMF19 is a novel PGIP gene that might be involved in pollen or tapetum development.