Characterization of a premeiotic germ cell-specific cytoplasmic protein encoded by Stra8, a novel retinoic acid-responsive gene.
Characterization of a premeiotic germ cell-specific cytoplasmic protein encoded by Stra8, a novel retinoic acid-responsive gene.
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DOI：
10.1083/jcb.135.2.469
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发表时间：
1996-10
影响因子：
7.8
通讯作者：
Chambon, P
中科院分区：
文献类型：
--
作者：
OuladAbdelghani, M;Bouillet, P;Decimo, D;Gansmuller, A;Heyberger, S;Dolle, P;Bronner, S;Lutz, Y;Chambon, P
Stra8 是一种可在 P19 胚胎癌细胞中由视黄酸 (RA) 诱导的新基因，其全长 cDNA 已被分离出来并显示编码 45 kD 的蛋白质。在用全反式和 9-顺式视黄酸处理的细胞中，Stra8 mRNA 和蛋白质均被诱导。二维凝胶分析和去磷酸化实验表明，RA 的两种立体异构体差异性地调节 Stra8 蛋白的磷酸化状态，该蛋白以不同的磷酸化形式存在。亚细胞分离和免疫细胞化学研究表明 Stra8 蛋白是细胞质的。在小鼠胚胎发生过程中，Stra8 的表达仅限于雄性发育中的性腺，而在成年小鼠中，Stra8 的表达仅限于减数分裂前的生殖细胞。因此，Stra8蛋白可能在精子发生的减数分裂前阶段发挥作用。
The full-length cDNA corresponding to Stra8, a novel gene inducible by retinoic acid (RA) in P19 embryonal carcinoma cells, has been isolated and shown to encode a 45-kD protein. Both Stra8 mRNA and protein were induced in cells treated by all-trans and 9-cis retinoic acids. Two- dimensional gel analysis and dephosphorylation experiments revealed that the two stereoisomers of RA differentially regulate the phosphorylation status of the Stra8 protein, which was shown to exist in differently phosphorylated forms. Subcellular fractionation and immunocytochemistry studies showed that the Stra8 protein is cytoplasmic. During mouse embryogenesis, Stra8 expression was restricted to the male developing gonads, and in adult mice, the expression of Stra8 was restricted to the premeiotic germ cells. Thus, Stra8 protein may play a role in the premeiotic phase of spermatogenesis.
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影响因子：
5.6
作者：
FYRBERG, E;FYRBERG, CC;SAVILLE, DL
通讯作者：
SAVILLE, DL
DOI：
10.1006/scel.1994.1015
发表时间：
1994-01-01
期刊：
Seminars in Cell Biology
影响因子：
--
作者：
Chambon, Pierre
通讯作者：
Chambon, Pierre
影响因子：
2.7
作者：
BOUILLET, P;OULADABDELGHANI, M;CHAMBON, P
通讯作者：
CHAMBON, P
影响因子：
64.5
作者：
DURAND, B;SAUNDERS, M;CHAMBON, P
通讯作者：
CHAMBON, P
影响因子：
64.5
作者：
HEYMAN, RA;MANGELSDORF, DJ;THALLER, C
通讯作者：
THALLER, C