Cyclic di-GMP signalling in the virulence and environmental adaptation of Xanthomonas campestris
Cyclic di-GMP signalling in the virulence and environmental adaptation of Xanthomonas campestris
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DOI：
10.1111/j.1365-2958.2006.05531.x
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发表时间：
2007-01-01
影响因子：
3.6
通讯作者：
Dow, J. Maxwell
中科院分区：
文献类型：
--
作者：
Ryan, Robert P.;Fouhy, Yvonne;Dow, J. Maxwell
环二GMP是第二信使，在多种细菌中的一系列细胞功能（包括病原体的毒力）的调节中发挥作用。细胞水平的环二GMP通过合成控制，由GGDEF蛋白结构域催化，并通过EAL或HD-GYP结构域降解。在这里，我们报告了一个全面的研究，在细菌性疾病中，我们研究的贡献，所有蛋白质与GGDEF，EAL或HD-GYP结构域的毒力和毒力因子生产的植物病原体野油菜黄单胞菌致病变种野油菜（XCC）。在对植物的毒力中具有重要作用的基因包括编码可能在环状二GMP合成中起作用的蛋白质的那些基因以及涉及环状二GMP降解的其他基因（包括HD-GYP结构域调节因子RpfG）。此外，RpfG控制这些基因的一个子集的表达。一组部分重叠的元件控制体外毒力因子的产生。其他GGDEF-EAL结构域蛋白对毒力因子合成没有影响，但确实影响运动性。这些研究结果表明，存在一个监管网络，可能允许XCC整合来自不同环境输入的信息，以调节毒力因子的合成，以及致力于其他特定任务的环二GMP信号系统。
Cyclic di-GMP is a second messenger with a role in regulation of a range of cellular functions in diverse bacteria including the virulence of pathogens. Cellular levels of cyclic di-GMP are controlled through synthesis, catalysed by the GGDEF protein domain, and degradation by EAL or HD-GYP domains. Here we report a comprehensive study of cyclic di-GMP signalling in bacterial disease in which we examine the contribution of all proteins with GGDEF, EAL or HD-GYP domains to virulence and virulence factor production in the phytopathogen Xanthomonas campestris pathovar campestris (Xcc). Genes with significant roles in virulence to plants included those encoding proteins whose probable function is in cyclic-di-GMP synthesis as well as others (including the HD-GYP domain regulator RpfG) implicated in cyclic di-GMP degradation. Furthermore, RpfG controlled expression of a subset of these genes. A partially overlapping set of elements controlled the production of virulence factors in vitro. Other GGDEF-EAL domain proteins had no effect on virulence factor synthesis but did influence motility. These findings indicate the existence of a regulatory network that may allow Xcc to integrate information from diverse environmental inputs to modulate virulence factor synthesis as well as of cyclic di-GMP signalling systems dedicated to other specific tasks.