Phage P22 lysis genes: nucleotide sequences and functional relationships with T4 and lambda genes.
Phage P22 lysis genes: nucleotide sequences and functional relationships with T4 and lambda genes.
复制标题
噬菌体 P22 裂解基因：核苷酸序列以及与 T4 和 lambda 基因的功能关系。
DOI：
10.1016/0042-6822(85)90115-1
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发表时间：
1985
期刊：
影响因子：
3.7
通讯作者：
Poteete,AR
中科院分区：
文献类型：
--
作者：
Rennell,D;Poteete,AR
对 P22 裂解基因的野生型和琥珀突变体等位基因进行了克隆和测序。 Gene13 编码一个 11,520 Da 的碱性疏水蛋白，与 λ S 蛋白具有 89% 的氨基酸同源性。 Gene19编码的蛋白质与T4溶菌酶具有小程度的氨基酸同源性，但我们无法检测到与λR或RZ蛋白质同源性。纯化19号基因的蛋白产物；其氨基末端氨基酸序列与DNA序列预测的一致。它以单个氨基末端甲硫氨酸残基开始，是一种分子量为 15,968 的碱性蛋白质。构建了表达P22基因19、λ基因RandRZ和T4基因的质粒。所有这些质粒都能够互补 λ R− 和 P22 19−。
Wild-type and amber mutant alleles of the lysis genes of P22 were cloned and sequenced. Gene13encodes an 11,520-Da basic hydrophobic protein that has 89% amino acid homology to λ S protein. Gene19encodes a protein that has a small degree of amino acid homology with T4 lysozyme, but we could detect no homology to λ R or RZ proteins. The protein product of gene19was purified; its amino terminal amino acid sequence is as predicted by the DNA sequence. It starts with a single amino terminal methionine residue and is a basic protein with a molecular weight of 15,968. Plasmids expressing P22 gene19, λ genesRandRZ, and T4 geneewere constructed. All of these plasmids were able to complement both λ R−and P22 19−.