DEHYDROASCORBATE UPTAKE AND REDUCTION BY HUMAN BLOOD NEUTROPHILS, ERYTHROCYTES, AND LYMPHOCYTES *
DEHYDROASCORBATE UPTAKE AND REDUCTION BY HUMAN BLOOD NEUTROPHILS, ERYTHROCYTES, AND LYMPHOCYTES *
复制标题
人血中性粒细胞、红细胞和淋巴细胞对脱氢坏血酸的吸收和减少 *
DOI：
10.1111/j.1749-6632.1975.tb29284.x
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发表时间：
1975
影响因子：
5.2
通讯作者：
R. Bigley
中科院分区：
文献类型：
--
作者：
L. Stankova;D. Rigas;R. Bigley
抗坏血酸可能作为细胞机制的一部分，灭活自由基和氧化剂，否则可能使细胞成分变性。‘-:’为了获得最大的效率，这种失活应该是酶促的。Green和O' brien已经证明，在抗坏血酸和肝微粒体或线粒体制剂存在的情况下，NADH可以减少脂质过氧化物。他们提出，半氢抗坏血酸还原酶[还原的NAD(P)：半氢抗坏血酸氧化还原酶，EC 1.6.5.4.1是该系统的一个组成部分。至少，抗坏血酸对氧化剂和自由基的有效失活需要酶对还原的抗坏血酸进行再生。为了确定脱氢抗坏血酸还原活性的组织差异是否可能与电离辐射和氧化应激敏感性的差异相关，我们测量了脱氢抗坏血酸的摄取和还原，以及C-1葡萄糖氧化的变化，在中性粒细胞、红细胞和淋巴细胞与脱氢抗坏血酸孵育期间。
Ascorbate may function as a part of cellular mechanisms for inactivating free radicals and oxidants that might otherwise denature cell constituents.'-:' For greatest efficiency, such inactivation should be enzymatic. Green and O'Brien have demonstrated reduction of lipid peroxides by NADH in the presence of ascorbate and liver microsomal or mitochondria1 preparations:' They propose that semidehydroascorbate reductase [reduced NAD( P) : semidehydroascorbate oxidoreductase, EC 1.6.5.4.1 is a component of this system. At the least, effective oxidant and free radical inactivation by ascorbate requires enzymatic regeneration of reduced ascorbate. To determine whether tissue differences in dehydroascorbate reducing activities might correlate with differences in sensitivity to ionizing radiation and oxidant stresses, we have measured dehydroascorbate uptake and reduction, along with changes in C-1 glucose oxidation, during incubation of neutrophils, erythrocytes, and lymphocytes with dehydroascorbate.