Biofilm-Sensitive Photodynamic Nanoparticles for Enhanced Penetration and Antibacterial Efficiency
Biofilm-Sensitive Photodynamic Nanoparticles for Enhanced Penetration and Antibacterial Efficiency
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生物膜敏感光动力纳米颗粒可增强渗透性和抗菌效率
DOI：
10.1002/adfm.202103591
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发表时间：
2021
影响因子：
19
通讯作者：
Fu-Jian Xu
中科院分区：
文献类型：
--
作者：
Shuangmei Wu;Chen Xu;Yiwen Zhu;Liang Zheng;Ludan Zhang;Yang Hu;Bingran Yu;Yuguang Wang;Fu-Jian Xu
由于细菌生物膜对传统药物的耐药性，迫切需要有效的抗菌剂来治疗细菌生物膜。光动力学疗法（PDT）是一种新的策略，已被用于对抗细菌和生物膜。通常选择阳离子光敏剂，特别是阳离子光动力纳米剂，以增强光动力抗微生物活性。然而，带正电荷的纳米颗粒（NP）有利于细胞内化，这导致细胞毒性增加。在此，设计了pH敏感的光动力纳米系统。用多粘菌素B（PMB）和葡萄糖酸（GA）以逐层方式修饰虎红（RB）聚多巴胺（PDA）NP，以产生功能适应性NP（RB@PMB@GA NP）。RB@PMB@GA NP在生理pH值下保持阴性，并表现出良好的生物相容性。当RB@PMB@GA NP暴露于酸性感染性环境时，由于pH敏感的静电相互作用，NP的表面电荷又带正电。这种表面电荷转换允许RB@PMB@GA有效地结合到细菌表面，并增强对革兰氏阴性细菌的光灭活效率。最重要的是，RB@PMB@GA NP在酸性条件下表现出良好的生物膜渗透和根除。此外，RB@PMB@GA NP在体内有效地消除生物膜感染。这项研究提供了一种有希望的策略，用于安全地治疗生物膜相关的体内感染。
Efficient antimicrobials are urgently needed for the treatment of bacterial biofilms due to their resistance to traditional drugs. Photodynamic therapy (PDT) is a new strategy that has been used to combat bacteria and biofilms. Cationic photosensitizers, particularly cationic photodynamic nanoagents, are usually chosen to enhance photodynamic antimicrobial activity. However, positively charged nanoparticles (NPs) are beneficial to cellular internalization, which causes increased cell cytotoxicity. Herein, a pH‐sensitive photodynamic nanosystem is designed. Rose Bengal (RB) polydopamine (PDA) NPs are decorated in a layer‐by‐layer fashion with polymyxin B (PMB) and gluconic acid (GA) to generate functionally adaptive NPs (RB@PMB@GA NPs). RB@PMB@GA NPs remain negative at physiological pH and exhibit good biocompatibility. When RB@PMB@GA NPs are exposed to an acidic infectious environment, the surface charge of the NPs is, in turn, positively charged as a result of pH‐sensitive electrostatic interactions. This surface charge conversion allows the RB@PMB@GA to effectively bind to the surfaces of bacteria and enhance photoinactivation efficiency against gram‐negative bacteria. Most importantly, RB@PMB@GA NPs exhibit good biofilm penetration and eradication under acidic conditions. Furthermore, RB@PMB@GA NPs efficiently eliminate biofilm infections in vivo. This study provides a promising strategy for safely treating biofilm‐associated infections in vivo.