Interspecific cross-hybrids of Nicotiana tabacum L. cv. (gla.) S 'K326' with Nicotiana alata
Interspecific cross-hybrids of Nicotiana tabacum L. cv. (gla.) S 'K326' with Nicotiana alata
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烟草种间杂交的研究
DOI：
10.1111/pbr.12474
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发表时间：
2017-06-01
期刊：
影响因子：
2
通讯作者：
Chen, Suiyun
中科院分区：
文献类型：
--
作者：
Liao, Jugou;Dai, Jinran;Chen, Suiyun
In order to introduce the Tomato Spotted Wilt Virus (TSWV) resistance from Nicotiana alata into Nicotiana tabacum, a cytoplasmic male sterility (CMS) line of N.tabacum (N.tabacum L. cv. (gla.) S 'K326'), was successfully crossed with N.alata. Despite a high DNA content variability, F-1 hybrids could be classified in two subgroups, a major one encompassing fertile hybrids morphologically similar to their tobacco maternal parent but TSWV sensitive, and a minor one displaying sterile hybrids showing an intermediate phenotype and TSWV resistant. In order to elucidate the unexpected fertility recovery of the fertile F-1 plants, some N.alata fertility restoration ppr genes were cloned and were shown to be differentially expressed between parental lineages as well as between both F-1 subgroups, suggesting that N.alata contains fertility restoring allele able to overcome the CMS of N.tabacum.
