Role of glutathione on acrylamide inhibition: Transformation products and mechanism
Role of glutathione on acrylamide inhibition: Transformation products and mechanism
复制标题
谷胱甘肽对丙烯酰胺抑制的作用：转化产物和机制
DOI：
10.1016/j.foodchem.2020.126982
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发表时间：
2020-10-01
期刊：
影响因子：
8.8
通讯作者：
Chen, Fang
中科院分区：
文献类型：
--
作者：
Zhu, Yuchen;Luo, Yinghua;Chen, Fang
谷胱甘肽(GSH)是加热食品中丙烯酰胺(AA)的潜在抑制剂。本研究在天冬酰胺(Asn)/葡萄糖(GLC)/GSH模型体系中研究了GSH对AA的抑制途径。与ASN/GLC模型体系相比，在ASN/GLC/GSH模型体系中确定了三个独特的分子离子(m/z470、379和193)。这些分子离子被确认为GLC与GSH反应生成的Amadori重排产物，以及AA与GSH与半胱氨酸(Cys)的巯基(-SH)的加成反应产物。ASN和GSH在竞争反应中对GLC的竞争可能是主要途径。此外，AA与GSH或AA与Cys之间的消除反应在AA的抑制作用中也起次要作用。前体、中间体和最终产物的差异为上述途径提供了定量证据。
Glutathione (GSH) is a potential inhibitor for acrylamide (AA) in heated food. In the present study, the inhibition pathways of GSH on AA were investigated in the asparagine (Asn)/glucose (Glc)/GSH model system. In comparison to the Asn/Glc model system, three unique molecular ions (m/z 470, 379, and 193) were identified in the Asn/Glc/GSH model system. Those molecular ions were confirmed as the Amadori rearrangement products which formed in the reaction between Glc and GSH, as well as the addition reaction products between AA and the sulfhydryl group (-SH) of GSH and cysteine (Cys). The competition between Asn and GSH for Glc in the competitive reactions was assumed to be the major pathway. Additionally, the elimination reaction between AA and GSH or between AA and Cys also played a minor role in the inhibition of AA. The variances of precursors, intermediates, and final products provided quantitative evidence for the above pathways.