Activation of the NaCl- and drought-induced RD29A and RD29B promoters by constitutively active Arabidopsis MAPKK or MAPK proteins (Retracted Article. See vol 29, pg 2253, 2006)
Activation of the NaCl- and drought-induced RD29A and RD29B promoters by constitutively active Arabidopsis MAPKK or MAPK proteins (Retracted Article. See vol 29, pg 2253, 2006)
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DOI：
10.1111/j.1365-3040.2006.01552.x
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发表时间：
2006-09-01
影响因子：
7.3
通讯作者：
Fromm, Michael E.
中科院分区：
文献类型：
--
作者：
Hua, Zhi-Ming;Yang, Xianci;Fromm, Michael E.
丝裂原活化蛋白（MAP）激酶介导细胞对各种刺激的反应。MAP激酶（MAPK）的活化发生在上游MAP激酶激酶（MAPKK）磷酸化之后。拟南芥基因组编码10个MKK，但其中很少有直接激活20个拟南芥MAPKs（AtMPKs）和NaCl-，干旱-或脱落酸（阿坝）诱导的基因RD 29 A或RD 29 B。我们构建了10种AtMKK蛋白中9种的组成型激活形式，并测试了它们激活RD 29 A和RD 29 B启动子的能力，还检查了9种激活的AtMKK蛋白在瞬时测定中磷酸化11种AtMPK蛋白的能力。结果表明，AtMKK 1、AtMKK 2和AtMKK 3三种蛋白可以激活RD 29 A启动子，而这三种和另外两种AtMKK 6/8蛋白可以激活RD 29 B启动子。利用瞬态分析，另外四种蛋白AtMKK 7/AtMKK 9和AtMKK 4/AtMKK 5可以引起烟草叶片中的过敏反应（HR）。RD 29 A启动子的激活与四种独特的激活AtMPK蛋白相关。一种新的方法激活AtMPK蛋白融合的顺式作用的突变体的人MAPK激酶MEK 1被用来确认的特定成员的AtMPK基因家族可以激活RD 29 A应激途径。
Mitogen-activated protein (MAP) kinases mediate cellular responses to a wide variety of stimuli. Activation of a MAP kinase (MAPK) occurs after phosphorylation by an upstream MAP kinase kinase (MAPKK). The Arabidopsis thaliana genome encodes 10 MKKs, but few of these have been shown directly to activate any of the 20 Arabidopsis MAPKs (AtMPKs) and NaCl-, drought- or abscisic acid (ABA)-induced genes RD29A or RD29B. We have constructed the constitutively activated form for nine of the 10 AtMKK proteins, and tested their ability to activate the RD29A and RD29B promoters and also checked the ability of the nine activated AtMKK proteins to phosphorylate 11 of the AtMPK proteins in transient assays. The results show that three proteins, AtMKK1, AtMKK2 and AtMKK3, could activate the RD29A promoter, while these three and two additional AtMKK6/8 proteins could activate the RD29B promoter. Four other proteins, AtMKK7/AtMKK9 and AtMKK4/AtMKK5, can cause hypersensitive response (HR) in tobacco leaves using transient analysis. The activation of the RD29A promoter correlated with four uniquely activated AtMPK proteins. A novel method of activating AtMPK proteins by fusion to a cis-acting mutant of a human MAPK kinase MEK1 was used to confirm that specific members of the AtMPK gene family can activate the RD29A stress pathway.