Formation of a thymus from rat ES cells in xenogeneic nude mouse⇆rat ES chimeras
Formation of a thymus from rat ES cells in xenogeneic nude mouse⇆rat ES chimeras
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DOI：
10.1111/j.1365-2443.2011.01495.x
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发表时间：
2011-04-01
期刊：
影响因子：
2.1
通讯作者：
Okabe, Masaru
中科院分区：
文献类型：
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作者：
Isotani, Ayako;Hatayama, Hide;Okabe, Masaru
胚胎干细胞(ES)或诱导多能干细胞(IPS)分化为特定类型的细胞系的各种条件正在深入研究中。然而，从ES或iPS细胞中产生具有三维结构的功能器官在体外很难实现。本研究建立了表达绿色荧光蛋白的大鼠ES细胞系，并将其注入小鼠胚泡，获得了小鼠ES嵌合体。大鼠ES细胞对嵌合体中的各种器官有贡献，包括生殖细胞。当我们将ES细胞注射到缺乏胸腺的nu/nu小鼠的囊胚中时，所产生的嵌合体产生了来自它们体内的大鼠ES细胞的胸腺。嵌合动物可能提供一种从ES或iPS细胞衍生各种器官的方法。
Various conditions for differentiating embryonic stem (ES) cells or induced pluripotent stem (iPS) cells into specific kinds of cell lines are under intensive investigation. However, the production of a functional organ with a three-dimensional structure from ES or iPS cells is difficult to achieve in vitro. In the present paper, we describe the establishment of a green fluorescent protein-expressing rat ES cell line and production of mouse rat ES chimera by injecting rat ES cells into mouse blastocysts. The rat ES cells contributed to various organs in the chimera, including germ cells. When we injected ES cells into blastocysts of nu/nu mice lacking a thymus, the resultant chimeras produced thymus derived from rat ES cells in their bodies. The chimeric animals may provide a method for the derivation of various organs from ES or iPS cells.