MODULATION OF MEMBRANE-FUNCTION BY CHOLESTEROL
MODULATION OF MEMBRANE-FUNCTION BY CHOLESTEROL
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DOI：
10.1016/0300-9084(91)90093-g
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发表时间：
1991-10-01
期刊：
影响因子：
3.9
通讯作者：
YEAGLE, PL
中科院分区：
文献类型：
--
作者：
YEAGLE, PL
胆固醇在哺乳动物（和其他需要胆固醇的）细胞中的重要作用的分子基础长期以来一直是人们强烈关注的对象。已经发现胆固醇调节对细胞功能至关重要的膜蛋白的功能。目前的文献支持这种调制的两种机制。在一种机制中，膜中高水平胆固醇的存在拮抗了作为其功能循环的一部分的整合膜蛋白对构象变化的“自由体积”的需求。在另一种机制中，固醇通过直接的固醇-蛋白质相互作用来调节膜蛋白的功能。这种机制提供了一个重要的膜蛋白的活性的刺激和细胞活力的结构特异性固醇的基本要求的解释。在某些情况下，这些后一种膜蛋白在不存在该细胞类型生长所需的特定固醇的情况下表现出很少或没有活性。所需的特定固醇因细胞类型而异，并且与该固醇影响膜的整体性质的能力无关。
The molecular basis for the essential role of cholesterol in mammalian (and other cholesterol-requiring) cells has long been the object of intense interest. Cholesterol has been found to modulate the function of membrane proteins critical to cellular function. Current literature supports two mechanisms for this modulation. In one mechanism, the requirement of 'free volume' by integral membrane proteins for conformational changes as part of their functional cycle is antagonized by the presence of high levels of cholesterol in the membrane. In the other mechanism, the sterol modulates membrane protein function through direct sterol-protein interactions. This mechanism provides an explanation for the stimulation of the activity of important membrane proteins and for the essential requirement of a structurally-specific sterol for cell viability. In some cases, these latter membrane proteins exhibit little or no activity in the absence of the specific sterol required for growth of that cell type. The specific sterol required varies from one cell type to another and is unrelated to the ability of that sterol to affect the bulk properties of the membrane.