Driving force of biomolecular liquid-liquid phase separation probed by nuclear magnetic resonance spectroscopy.
Driving force of biomolecular liquid-liquid phase separation probed by nuclear magnetic resonance spectroscopy.
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核磁共振波谱探测生物分子液-液相分离驱动力
DOI：
10.52601/bpr.2022.210034
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发表时间：
2022-04-30
影响因子：
--
通讯作者：
Ruan K
中科院分区：
文献类型：
--
作者：
Zhang H;Fan W;Nshogoza G;Liu Y;Gao J;Wu J;Shi Y;Tu X;Zhang J;Ruan K
生物分子凝聚体的组装是由液-液相分离驱动的。为了理解这些冷凝物的结构和功能，必须表征潜在的驱动力，例如，蛋白质-蛋白质和蛋白质-RNA相互作用。由于相分离过程涉及结构域和低复杂度域，NMR可能是唯一可以同时用于描述结构域和非结构域的结合拓扑结构和相互作用模式的技术。任何一对组分之间的分子内和分子间相互作用的原子分辨率分析揭示了相分离和生物分子冷凝物组装和拆卸的机制。在这里，我们描述了用于最广泛采用的NMR技术来表征生物分子相分离的关键相互作用的程序。
The assembly of biomolecular condensates is driven by liquid–liquid phase separation. To understand the structure and functions of these condensates, it is essential to characterize the underlying driving forces, e.g., protein–protein and protein–RNA interactions. As both structured and low-complexity domains are involved in the phase separation process, NMR is probably the only technique that can be used to depict the binding topology and interaction modes for the structured and nonstructured domains simultaneously. Atomic-resolution analysis for the intramolecular and intermolecular interactions between any pair of components sheds light on the mechanism for phase separation and biomolecular condensate assembly and disassembly. Herein, we describe the procedures used for the most extensively employed NMR techniques to characterize key interactions for biomolecular phase separation.