Highly similar piggyBac transposase-like sequences from various Bactrocera (Diptera, Tephritidae) species
Highly similar piggyBac transposase-like sequences from various Bactrocera (Diptera, Tephritidae) species
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DOI：
10.1111/j.1365-2583.2007.00756.x
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发表时间：
2007-10-01
影响因子：
2.6
通讯作者：
Gasperi, G.
中科院分区：
文献类型：
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作者：
Bonizzoni, M.;Gomulski, L. M.;Gasperi, G.
piggyBac转座因子是目前在昆虫和哺乳动物中用于转基因、增强子捕获、基因发现和基因功能测定的首选载体。然而，最近在多种生物中发现的与piggyBac相似的序列表明，piggyBac可能会水平转移到远亲物种中。这引起了人们对piggyBac转化载体的广泛应用及其稳定性的关注。在本文中，piggyBac转座酶的同源序列的存在下进行了调查，在17种属于6属的实蝇科，包括许多害虫物种的转化已经实现。piggyBac样序列，与原始粉纹夜蛾转座酶序列的高度相似性，仅在实蝇属的六个物种中被鉴定。
The piggyBac transposable element is currently the vector of choice for transgenesis, enhancer trapping, gene discovery and gene function determination in both insects and mammals. However, the recent discovery of sequences with similarity to piggyBac in a wide diversity of organisms suggests that piggyBac may be horizontally transferred to distantly related species. This has raised concern on the wide-range application of piggyBac-based transformation vectors and their stability. In this paper, the presence of sequences homologous to the piggyBac transposase was investigated in 17 species belonging to six genera within the Tephritidae family, including many pest species for which transformation has already been achieved. piggyBac-like sequences, with a high degree of similarity to the original Trichoplusia ni transposase sequence were identified only in six species of the Bactrocera genus.