CELL-SURFACE ANTIGENS OF HUMAN MALIGNANT-MELANOMA - MIXED HEMADSORPTION ASSAYS FOR HUMORAL IMMUNITY TO CULTURED AUTOLOGOUS MELANOMA CELLS
CELL-SURFACE ANTIGENS OF HUMAN MALIGNANT-MELANOMA - MIXED HEMADSORPTION ASSAYS FOR HUMORAL IMMUNITY TO CULTURED AUTOLOGOUS MELANOMA CELLS
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DOI：
10.1073/pnas.73.9.3278
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发表时间：
1976-01-01
影响因子：
11.1
通讯作者：
OLD, LJ
中科院分区：
文献类型：
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作者：
CAREY, TE;TAKAHASHI, T;OLD, LJ
通过混合血液吸附试验，研究了恶性黑色素瘤患者对培养的自体黑色素瘤细胞表面抗原产生特异性抗体的频率。35例II期、III期和IV期患者的研究时间从1-36个月不等，其中2例对自体黑色素瘤细胞表现出一致的高滴度反应性，2例表现出不太一致的中等反应性，7例表现出零星的低滴度反应性，其余均为阴性。对1例患者的血清进行了详细的分析，该血清对自体黑色素瘤细胞具有足够高的滴度。通过对多种黑色素瘤和非黑色素瘤细胞系（包括人黑色素瘤SK-Mel-1（及其衍生物）和RPMI-7931细胞、人膀胱癌J-82和T-24细胞、人淋巴母细胞SK-L-7细胞和人肾癌SK-RC-1和SK-RC-4细胞）（包括自体成纤维细胞）的直接试验和吸收分析，该抗原不能在除自体黑色素瘤以外的任何细胞类型上证明。
How frequently patients with malignant melanoma have specific antibody to cell surface antigens of cultured autologous melanoma cells as demonstrated by mixed hemadsorption assays was studied. Of 35 patients studied over periods ranging from 1-36 mo. with Stage II, III and IV disease, 2 showed consistent and high titered reactivity against autologous melanoma cells, 2 showed less consistent and intermediate reactivity, 7 showed sporadic, low titered reactivity, and the remainder were consistently negative. A detailed analysis was carried out with the sera of 1 patient with sufficiently high titer against autologous melanoma cells. By direct tests and by absorption analysis with a variety of melanoma and nonmelanoma cell lines [including human melanoma SK-Mel-1 (and derivatives) and RPMI-7931 cells, human bladder carcinoma J-82 and T-24 cells, human lymphoblast SK-L-7 cells and human renal carcinoma SK-RC-1 and SK-RC-4 cells] which included autologous fibroblasts, the antigen could not be demonstrated on any cell type other than the autologous melanoma.