Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C-elegans
Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C-elegans
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DOI：
10.1101/gad.927801
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发表时间：
2001-10-15
影响因子：
10.5
通讯作者：
Plasterk, RHA
中科院分区：
文献类型：
--
作者：
Ketting, RF;Fischer, SEJ;Plasterk, RHA
双链RNA可以通过一种进化上保守的过程抑制同源基因的表达，称为RNA干扰（RNAi）或转录后基因沉默（PTGS）。沉默的一个潜在机制是通过RNP复合物降解靶mRNA，该复合物含有类似于22 nt的siRNA作为底物选择的指导。称为Dicer的双齿核酸酶被认为是负责siRNA产生的蛋白质。在这里，我们的特点秀丽隐杆线虫直向同源的Dicer（K12H4.8; DCR-1）在体内和体外。dcr-1突变体在RNA干扰中表现出缺陷。此外，表型异常和RNA分析的组合表明dcr-1在由小时间RNA（let-7）及其靶标（例如，lin-41）。
Double-stranded RNAs can suppress expression of homologous genes through an evolutionarily conserved process named RNA interference (RNAi) or post-transcriptional gene silencing (PTGS). One mechanism underlying silencing is degradation of target mRNAs by an RNP complex, which contains similar to 22 nt of siRNAs as guides to substrate selection. A bidentate nuclease called Dicer has been implicated as the protein responsible for siRNA production. Here we characterize the Caenorhabditis elegans ortholog of Dicer (K12H4.8; dcr-1) in vivo and in vitro. dcr-1 mutants show a defect in RNAi. Furthermore, a combination of phenotypic abnormalities and RNA analysis suggests a role for dcr-1 in a regulatory pathway comprised of small temporal RNA (let-7) and its target (e.g., lin-41).