Polarographic needle electrode measurements of oxygen in rat prostate carcinomas: accuracy and reproducibility.
Polarographic needle electrode measurements of oxygen in rat prostate carcinomas: accuracy and reproducibility.
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大鼠前列腺癌中氧的极谱针电极测量：准确性和再现性。
DOI：
10.1016/0360-3016(95)00036-x
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Chapman,DC
中科院分区：
文献类型：
--
作者：
Yeh,KA;Biade,S;Lanciano,RM;Brown,DQ;Fenning,MC;Babb,JS;Hanks,GE;Chapman,DC
目的肿瘤的氧合状态对于预测肿瘤对治疗的反应可能很重要。先前使用肿瘤氧合间接测定对间变性（R3327-AT）和分化良好（R3327-H）邓宁大鼠前列腺肿瘤进行的研究表明，间变性肿瘤具有相对缺氧和抗辐射的性质。我们现在报告使用 pO 2 直方图对这些肿瘤中的氧进行直接测量，以确定：(a) 是否可以检测到它们之间氧合状态的显着差异；(b) 对同一肿瘤的连续测量是否给出相似的值； (c)肿瘤氧合是否与肿瘤体积相关。 方法和材料R3327-AT和R3327-H肿瘤在Fischer X哥本哈根大鼠中生长至1.0-7.0cm 3 的体积。沿着两条平行轨道在麻醉动物的肿瘤中进行电极测量(100-200)。 1-5 天沿不同的平行轨道重复测量。测量肌肉组织的氧分压并作为正常组织对照。应用统计分析来确定两种肿瘤组织学之间的肿瘤氧水平是否不同，同一肿瘤中的连续测量是否可重复，以及肿瘤氧合是否与肿瘤体积相关。结果分化良好（n = 15）和间变性（n = 15）肿瘤的平均中位pO 2 分别为6.0 mmHg（SE+/-1.3）和2.2 mmHg（SE+/-0.3）。正常大鼠肌肉（n=15）的平均中位pO 2 为23.6mmHg（SE+/-2.0）。这些值代表两个肿瘤和大鼠肌肉之间氧浓度的高度显着差异。平均 pO 2 值的差异也非常显着。在不同日期对同一肿瘤进行重复测量，R3327-H (n= 15) 和 R3327-AT (n= 15) 肿瘤的平均中值分别为 4.7 和 2.2 mmHg。对于这些重复测量，15 个肿瘤的中位 pO 2 值下降，15 个肿瘤的中位 pO 2 值增加，并且与第一次测量没有显着差异。 R3327-H 和 R3327-AT 肿瘤的中位 pO 2 观察到的平均差异分别为 37%(SE+/-7) 和 58%(SE+/-10)。本研究中未观察到pO 2 水平与肿瘤体积之间存在显着相关性。结论间变性Dunning 肿瘤的中位pO 2 值显着低于分化良好的肿瘤（p<0.001）。两种肿瘤中的氧水平均显着低于正常大鼠肌肉中测量的氧水平（p<0.00005）。对于任一肿瘤模型，同一肿瘤中中位 pO 2 的重复测量没有显着差异 (p> 0.5)。通过仅沿两个插入位点采样，每天观察到的单个肿瘤内 pO 2 分布的变化可能表明其氧合状态的真实动态和/或电极测量的限制。这些肿瘤模型中 pO 2 的电极测量是可重复的，并证实了先前检测到的间变性肿瘤和分化良好的肿瘤之间的氧合差异。
PurposeThe oxygenation status of tumors may be important for predicting tumor response to therapy. Previous studies with the anaplastic (R3327-AT) and well-differentiated (R3327-H) Dunning rat prostate tumors using indirect assays of tumor oxygenation indicated the relative hypoxic and radioresistant nature of the anaplastic tumor. We now report direct measurements of oxygen in these tumors made with the pO 2 histograph to determine:(a) whether a significant difference in oxygenation status could be detected between them;(b) whether sequential measurements on the same tumor gave similar values; and (c) whether tumor oxygenation correlated with tumor volume.Methods and MaterialsR3327-AT and R3327-H tumors were grown in Fischer X Copenhagen rats to volumes of 1.0-7.0 cm 3. Electrode measurements (100-200) were made in tumors in anesthetized animals along two parallel tracks. Repeat measurements were made at 1-5 days along different parallel tracks. Oxygen partial pressures of muscle tissue were measured and served as a normal tissue control. Statistical analyses were applied to determine whether tumor oxygen levels were different between the two tumor histologies, whether sequential measurements in the same tumor were reproducible, and whether tumor oxygenation correlated with tumor volume.ResultsThe average median pO 2 of the well-differentiated (n= 15) and the anaplastic (n= 15) tumors was 6.0 mmHg (SE+/-1.3) and 2.2 mmHg (SE+/-0.3), respectively. The average median pO 2 of normal rat muscle (n= 15) was 23.6 mmHg (SE+/-2.0). These values represent highly significant differences in oxygen concentration between the two tumors and rat muscle. The differences in average mean pO 2 values were also highly significant. Repeat measurements in the same tumors on different days gave average median values of 4.7 and 2.2 mmHg in the R3327-H (n= 15) and R3327-AT (n= 15) tumors, respectively. For these repeat measurements, median pO 2 values decreased in 15 and increased in 15 tumors, and were not significantly different from the first measurements. The average differences observed in median pO 2 were 37%(SE+/-7) and 58%(SE+/-10) for the R3327-H and R3327-AT tumors, respectively. No significant correlation was observed between pO 2 levels and the tumor volumes investigated in this study.ConclusionsThe median pO 2 values of the anaplastic Dunning tumors were significantly lower than those of the well-differentiated tumors (p< 0.001). Oxygen levels in both tumors were significantly lower than those measured in normal rat muscle (p< 0.00005). Repeat measurements of median pO 2 in the same tumors were not significantly different for either tumor model (p> 0.5). The changes observed in pO 2 distributions within individual tumors from day to day may indicate true dynamics of its oxygenation status and/or the limits of electrode measurements, by sampling along only two insertion sites. The electrode measurements of pO 2 in these tumor models are reproducible and confirm previously detected oxygenation differences between the anaplastic and well-differentiated tumors.