Evidence for vagal enkephalinergic neural control of the feline pylorus and stomach.
Evidence for vagal enkephalinergic neural control of the feline pylorus and stomach.
复制标题
迷走神经脑啡肽能神经控制猫幽门和胃的证据。
DOI：
10.5555/uri:pii:0016508580908628
复制
发表时间：
1980
期刊：
影响因子：
29.4
通讯作者：
H. Ahlman
中科院分区：
文献类型：
--
作者：
R. Edin;J. Lundberg;L. Terenius;A. Dahlström;T. Hökfelt;J. Kewenter;H. Ahlman
用一种新的经幽门血流测定方法，研究了猫幽门括约肌对阿托品和乙啶的抵抗性收缩。经动脉注入阿片受体拮抗剂纳洛酮可剂量依赖性地阻断幽门收缩。局部动脉注射吗啡或脑啡酰胺引起幽门和胃收缩。亮氨酸和甲硫氨酸脑啡肽动脉内引起幽门收缩和胃舒张。纳洛酮可预防或逆转上述作用。免疫组织化学研究显示，特别丰富的脑啡肽能神经支配的幽门与静脉曲张的神经纤维与脑啡肽样免疫反应，在环形平滑肌层和肌间神经丛的密集网络。此外，许多肌间神经丛的细胞体与脑啡肽样物质被证明。脑啡肽样神经支配的胃体-胃窦环肌层稀疏，肌间神经丛中仅观察到少量免疫反应阳性细胞体。这些结果共同表明迷走神经控制猫幽门和胃介导的脑啡肽能神经元。
The atropine- and guanethidine-resistant contraction of the cat pyloric sphincter induced by efferent stimulation of the cervical vagi, was studied with a new method for measuring transpyloric flow. The pyloric contraction was blocked in a dose-dependent manner by the opiate antagonist naloxone infused intraarterially to the pyloric region. Local intraarterial injection of morphine or enkephalinamide elicited a pyloric and gastric contraction. Leu- and met-enkephalin intraarterially elicited a pyloric contraction and a gastric relaxation. These effects could be prevented or reversed by naloxone. Immunohistochemical studies revealed a particularly rich enkephalinergic innervation of the pylorus with dense networks of varicose nerve fibers with enkephalin-like immunoreactivity both in the circular smooth muscle layer and in the myenteric plexus. Furthermore, many cell bodies of the myenteric plexus with enkephalin-like material were demonstrated. The corpus-antrum was sparsely innervated by enkephalin-like nerves to the circular muscle layer and only few immunoreactive cell bodies were observed in the myenteric plexus. These results together suggest a vagal control of the feline pylorus and stomach mediated via enkephalinergic neurons.