Pretreatment Affects Profits From Xylanase During Enzymatic Saccharification of Corn Stover Through Changing the Interaction Between Lignin and Xylanase Protein.
Pretreatment Affects Profits From Xylanase During Enzymatic Saccharification of Corn Stover Through Changing the Interaction Between Lignin and Xylanase Protein.
复制标题
预处理通过改变木质素和木聚糖酶蛋白之间的相互作用影响玉米秸秆酶糖化过程中木聚糖酶的利润
DOI：
10.3389/fmicb.2021.754593
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发表时间：
2021
影响因子：
5.2
通讯作者：
Qu Y
中科院分区：
文献类型：
--
作者：
Feng X;Yao Y;Xu N;Jia H;Li X;Zhao J;Chen S;Qu Y
有效的预处理对于提高生物质转化效率至关重要，这通常需要添加木聚糖酶作为辅助酶来增强玉米秸秆的酶促糖化。在这项研究中，我们研究了亚硫酸铵（AS）和蒸汽爆炸（SE）两种复杂的预处理方法对玉米秸秆酶解中木聚糖酶利润的影响。我们进一步探讨了木质素和木聚糖酶 Xyn10A 蛋白之间的相互作用。我们的结果表明，添加 Xyn10A 的 AS 预处理玉米秸秆中葡聚糖和木聚糖的转化率高于 SE 预处理的玉米秸秆中葡聚糖和木聚糖的转化率。与SE预处理玉米秸秆中的木质素相比，AS预处理玉米秸秆中的木质素具有较低的Xyn10A初始吸附速度（13.56 vs. 10.89 mg g−1 min−1）和吸附容量（49.46 vs. 27.42 mg g−1木质素）和减弱的结合强度（310.6 vs. 215.9 L g−1）。我们的研究表明，木质素的低绝对zeta电位和强亲水性可能部分解释了木聚糖酶蛋白与来自AS预处理的玉米秸秆的木质素之间相对较弱的相互作用。总之，我们的结果表明，AS预处理减弱了木质素对酶的抑制，促进了玉米秸秆的酶促水解，降低了生物转化中酶的成本。
Effective pretreatment is vital to improve the biomass conversion efficiency, which often requires the addition of xylanase as an accessory enzyme to enhance enzymatic saccharification of corn stover. In this study, we investigated the effect of two sophisticated pretreatment methods including ammonium sulfite (AS) and steam explosion (SE) on the xylanase profits involved in enzymatic hydrolysis of corn stover. We further explored the interactions between lignin and xylanase Xyn10A protein. Our results showed that the conversion rates of glucan and xylan in corn stover by AS pretreatment were higher by Xyn10A supplementation than that by SE pretreatment. Compared with the lignin from SE pretreated corn stover, the lignin from AS pretreated corn stover had a lower Xyn10A initial adsorption velocity (13.56 vs. 10.89 mg g−1 min−1) and adsorption capacity (49.46 vs. 27.42 mg g−1 of lignin) and weakened binding strength (310.6 vs. 215.9 L g−1). Our study demonstrated the low absolute zeta potential and strong hydrophilicity of the lignin may partly account for relative weak interaction between xylanase protein and lignin from AS pretreated corn stover. In conclusion, our results suggested that AS pretreatment weakened the inhibition of lignin to enzyme, promoted the enzymatic hydrolysis of corn stover, and decreased the cost of enzyme in bioconversion.