Neurologic evaluation of acute lacrimomimetic effect of cyclosporine in an experimental rabbit dry eye model.
Neurologic evaluation of acute lacrimomimetic effect of cyclosporine in an experimental rabbit dry eye model.
复制标题
环孢素在实验兔干眼模型中急性拟泪作用的神经学评估。
DOI：
10.1167/iovs.08-1880
复制
发表时间：
2009
影响因子：
4.4
通讯作者：
Murakami,Akira
中科院分区：
文献类型：
--
作者：
Toshida,Hiroshi;Nguyen,DoanH;Beuerman,RogerW;Murakami,Akira
方法。正常成年雄性新西兰大白兔和接受副交感神经切除的新西兰大白兔均接受0.1% CsA或载药滴眼液的单次滴注。分别在注射CsA或载药前后测量泪滴试验（STT）结果、泪腺（LG）液从泪腺主泪道排出的流速和眨眼率（3分钟内）。光镜下观察体外主要LG。用CsA孵育30分钟后，测定LG片段的蛋白释放。正常家兔经CsA处理后的STT值和LG液流速较对照显著升高（P< 0.05）。相比之下，在失神经的眼睛中没有发现任何变化。CsA处理后正常家兔眼眨眼率显著高于载药处理（P< 0.005），无神经支配的家兔眼眨眼率显著低于给药前（P< 0.005）。光镜观察显示，副交感神经切除后7天，无神经支配的LG组织中腺泡细胞细胞质中充满分泌颗粒。CsA滴注3小时后观察到同样的结果。CsA对不同浓度的LG片段中腺泡细胞的蛋白释放均无刺激作用。这些结果表明，CsA对急性模型LG泪液分泌无直接影响。相反，CsA增加反射性泪流。
methods. Normal adult male New Zealand White rabbits and those that underwent parasympathectomy each received a single instillation of 0.1% CsA or vehicle eyedrops. Schirmer tear test (STT) results, flow rate of lacrimal gland (LG) fluid from the excretory lacrimal duct of the main LG, and blink rate (over a 3-minute period) were measured before and after instillation of CsA or vehicle. Light microscopy was performed to examine the main LG in vitro. Protein release from LG fragments was assessed after incubation with CsA for 30 minutes.results. In normal rabbits, the STT value and the flow rate of LG fluid were significantly increased after treatment with CsA compared with vehicle (P< 0.05). In contrast, no changes were found in denervated eyes. The blink rate of CsA-treated eyes was significantly higher than that of vehicle-treated eyes in normal rabbits (P< 0.005), whereas that of denervated eyes decreased significantly after CsA instillation compared with before administration (P< 0.005). Light microscopy showed that the cytoplasm of acinar cells was packed with secretory granules in denervated LG tissue 7 days after parasympathectomy. The same finding was observed 3 hours after CsA instillation. CsA had no stimulatory effect on protein release by acinar cells in LG fragments at all concentrations tested.conclusions. These results suggest that CsA has no direct effect on tear fluid secretion from the LG in an acute model. Instead, CsA increases reflex tear flow.