Enhanced binding of neuraminidase-treated sheep erythrocytes to human T lymphocytes.
Enhanced binding of neuraminidase-treated sheep erythrocytes to human T lymphocytes.
复制标题
神经氨酸酶处理的绵羊红细胞与人 T 淋巴细胞的结合增强。
DOI：
10.1182/blood.v42.6.939.939
复制
发表时间：
1973
期刊：
影响因子：
20.3
通讯作者：
Victor Nussenzweig
中科院分区：
文献类型：
--
作者：
Michael S. Weiner;C. Bianco;Victor Nussenzweig
Pretreatment of sheep erythrocytes with neuraminidase greatly enhances their specific binding and rosette formation with human T lymphocytes. Since the rosettes dissociate at 37°C, a method was developed to isolate human T lymphocytes with a high degree of purity and good recoveries. Trypsin treatment of E abolishes their binding to T lymphocytes. Trypsin-treated erythrocytes can be coated with antibody and complement components and used as a specific reagent to detect the C3 receptor on human B lymphocytes, avoiding the risk of an interaction between the erythrocytes and T lymphocytes.
