Immunomodulation produced by a green propolis extract on humoral and cellular responses of mice immunized with SuHV-1
Immunomodulation produced by a green propolis extract on humoral and cellular responses of mice immunized with SuHV-1
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DOI：
10.1016/j.vaccine.2006.10.005
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发表时间：
2007-01-26
期刊：
影响因子：
5.5
通讯作者：
Vidor, Telmo
中科院分区：
文献类型：
--
作者：
Fischer, Geferson;Conceicao, Fabricio Rochedo;Vidor, Telmo
尽管最近疫苗生产的技术进步，大多数疫苗依赖于与佐剂物质的关联。本研究评价了绿色芦荟乙醇提取物对灭活猪疱疹病毒1型（SuHV-1）疫苗制剂的佐剂作用。接种SuHV-1疫苗加氢氧化铝和5 mg/剂量的黄芪提取物的小鼠，与接受相同疫苗但未接种黄芪的动物相比，抗体水平更高。单独使用SuHV-1疫苗和黄芪提取物没有诱导显著水平的抗体，但是它能够增加细胞免疫应答，这通过IFN-γ mRNA表达的增加来证明。此外，该疫苗还提高了受保护动物对致死剂量SuHV-1攻击的抵抗率。绿色枸杞提取物对免疫和细胞免疫反应的影响可用于开发有效的疫苗。(c)2006爱思唯尔有限公司版权所有。
Despite recent technological advances in vaccine production, most the vaccines depend on the association with adjuvant substances. This work evaluated the adjuvant capacity of an ethanol extract of green propolis associated to inactivated Suid herpesvirus type 1 (SuHV-1) vaccine preparations. Mice inoculated with SuHV-1 vaccine plus aluminum hydroxide and 5 mg/dose of propolis extract presented higher levels of antibodies when compared to animals that received the same vaccine without propolis. The use of SuHV-1 vaccine with propolis extract alone did not induce significant levels of antibodies, however it was able to increase the cellular immune response, evidenced by the increase in the expression of mRNA to IFN-gamma. Besides, propolis increased the percentage of protected animals against challenge with a lethal dose of SuHV-1. The effect of green propolis extract on the Immoral and cellular immune responses may be exploited for the development of effective vaccines. (c) 2006 Elsevier Ltd. All rights reserved.