Momilactones A and B Are α-Amylase and α-Glucosidase Inhibitors
Momilactones A and B Are α-Amylase and α-Glucosidase Inhibitors
复制标题
DOI：
10.3390/molecules24030482
复制
发表时间：
2019-02-01
期刊：
影响因子：
4.6
通讯作者：
Teschke, Rolf
中科院分区：
文献类型：
--
作者：
Nguyen Van Quan;Hoang-Dung Tran;Teschke, Rolf
苦瓜内酯A（MA）和B（MB）是水稻中具有活性的植物抗毒素和化感物质。本研究从水稻（Oryza sativa cv.）通过柱色谱法从Koshihikari中分离纯化，并通过高效液相色谱法、薄层色谱法、气相色谱-质谱法、液相色谱-电喷雾电离-质谱法（LC-ESI-MS）以及H-1和C-13核磁共振分析确认纯化。通过体外试验，MA和MB都对α-淀粉酶和α-葡萄糖苷酶活性产生了有效的抑制作用。MB对这两种关键酶的抑制作用大于MA。MA和MB都表现出比商业糖尿病抑制剂阿卡波糖更强的α-葡萄糖苷酶抑制作用。水稻籽粒中MA和MB的平均含量分别为2.07 ± 0.01和1.06 ± 0.01 μ g/干重。本研究首次证实了精制稻米中MA和MB的存在，并报道了这两种化合物的α-淀粉酶和α-葡萄糖苷酶抑制活性。本研究改进的LC-ESI-MS方法简便、有效，可用于水稻器官中MA和MB的检测和分离。
Momilactones A (MA) and B (MB) are the active phytoalexins and allelochemicals in rice. In this study, MA and MB were purified from rice husk of Oryza sativa cv. Koshihikari by column chromatography, and purification was confirmed by high-performance liquid chromatography, thin-layer chromatography, gas chromatography-mass spectrometry, liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS), and H-1 and C-13 nuclear magnetic resonance analyses. By in vitro assays, both MA and MB exerted potent inhibition on alpha-amylase and alpha-glucosidase activities. The inhibitory effect of MB on these two key enzymes was greater than that of MA. Both MA and MB exerted greater alpha-glucosidase suppression as compared to that of the commercial diabetic inhibitor acarbose. Quantities of MA and MB in rice grain were 2.07 +/- 0.01 and 1.06 +/- 0.01 mu g/dry weight (DW), respectively. This study was the first to confirm the presence of MA and MB in refined rice grain and reported the alpha-amylase and alpha-glucosidase inhibitory activity of the two compounds. The improved protocol of LC-ESI-MS in this research was simple and effective to detect and isolate MA and MB in rice organs.