Lignofibrils on the external cell wall surface of cultured plant cells.
Lignofibrils on the external cell wall surface of cultured plant cells.
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DOI：
10.1083/jcb.50.1.63
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发表时间：
1971-07
影响因子：
7.8
通讯作者：
Martin, S M
中科院分区：
文献类型：
--
作者：
Leppard, G G;Colvin, J R;Rose, D;Martin, S M
小链和链束从悬浮培养的胡萝卜、红薯和菜豆细胞的外表面延伸到培养基中。这种纤维细胞外套存在于各个生长阶段的所有样品中，但数量似乎按红薯 < 菜豆 < 胡萝卜的顺序增加。这些束的长度通常为数微米，并且在频率、尺寸和形状方面表现出很大的变化。将线束和束的组成鉴定为木质素与以下观察结果一致：将线束碱性硝基苯氧化成类似于木材木质素单体的化合物；链对链霉蛋白酶、胰蛋白酶、果胶酶和脂肪酶的抗性；对重金属有很强的不可逆吸附作用；用硝酸银-六胺试剂处理沉积银颗粒；用二恶烷水溶液萃取束（Björkman 程序）；大量存在结构化形式的克拉森木质素；以及存在使用威斯纳试剂进行阳性测试的材料。来自菜豆的大的单条线（木质原纤维）呈扁平带状，具有不规则间隔的非常细的分支。这种形式不随制备技术的变化而变化，尽管其电子不透明性会变化。细胞间隙显示出相当大的结构，有时包含与细胞之间的中间层和面向介质的表面束融合的纤维状材料片。这些片可能是木质原纤维的另一种结合形式。这表明天然纤维木质素可能是植物组织中比迄今为止所怀疑的更常见的成分。
Small strands and bundles of strands extend from the outside surface of suspension-cultured cells of Daucus, Ipomoea, and Phaseolus into the medium. This fibrous cell coat is present in all samples from various growth stages but appears to increase in quantity in the order Ipomoea < Phaseolus < Daucus. The bundles are often many microns in length and display great variation in frequency, size, and form. Identification of the composition of the strands and bundles as lignin is consistent with the following observations: alkaline nitrobenzene oxidation of the strands to compounds which resemble monomers of wood lignin; resistance of the strands to pronase, trypsin, pectinase, and lipase; strong irreversible adsorption of heavy metals; deposition of silver granules by treatment with silver nitrate-hexamine reagent; extraction of the bundles with aqueous dioxane (Björkman procedure); presence in quantity of a structured form of Klason lignin; and existence of material giving a positive test with the Wiesner reagent. Large individual strands (lignofibrils) from Phaseolus show the form of a flat ribbon with very thin branches at irregular intervals. This form does not vary with preparatory techniques, although its electron opacity does. Intercellular spaces display considerable structure and sometimes contain sheets of fibrillar material merging with both the middle lamella between the cells and the surface bundles facing the medium. These sheets are probably another form of association of the lignofibrils. It is suggested that natural fibrous lignin may be a much commoner component of plant tissue than suspected hitherto.