Protein-RNA interactions in the active center of transcription elongation complex.
Protein-RNA interactions in the active center of transcription elongation complex.
复制标题
转录延伸复合物活性中心的蛋白质-RNA 相互作用。
DOI：
10.1073/pnas.93.8.3221
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发表时间：
1996
影响因子：
11.1
通讯作者：
Goldfarb,A
中科院分区：
文献类型：
--
作者：
Markovtsov,V;Mustaev,A;Goldfarb,A
通过将交联探针插入新生转录物的3'端，在大肠杆菌RNA聚合酶中定位了三个位点，这些位点与生产延伸复合物中的RNA接触。其中两个位点在β亚基上一个在β '亚基上。在延伸过程中，转录复合体偶尔会发生阻滞，因此它既不能延伸也不能释放RNA转录物。结果表明，在阻滞复合物中，RNA 3‘末端的三个接触丢失，而一个新的β ’亚基接触变得突出。因此，延长阻滞似乎涉及到大部分活性中心从RNA的3'末端脱离，并将末端转移到新的蛋白质环境中。
By using a crosslinkable probe incorporated into the 3' terminus of nascent transcript, three sites were mapped in Escherichia coli RNA polymerase that are contacted by the RNA in the productive elongation complex. Two of these sites are in the beta subunit and one is in the beta' subunit. During elongation, the transcription complex occasionally undergoes an arrest whereby it can neither extend nor release the RNA transcript. It is demonstrated that in an arrested complex, the three contacts of RNA 3' terminus are lost, while a new beta' subunit contact becomes prominent. Thus, elongation arrest appears to involve the disengagement of the bulk of the active center from the 3' terminus of RNA and the transfer of the terminus into a new protein environment.
DOI：
--
发表时间：
1994
期刊：
影响因子：
--
作者：
R. Conaway;J. Conaway
通讯作者：
J. Conaway