Natural variation in stochastic photoreceptor specification and color preference in Drosophila
Natural variation in stochastic photoreceptor specification and color preference in Drosophila
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DOI：
10.7554/elife.29593
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发表时间：
2017-12-18
期刊：
影响因子：
7.7
通讯作者：
Johnston, Robert J.
中科院分区：
文献类型：
--
作者：
Anderson, Caitlin;Reiss, India;Johnston, Robert J.
每个人都以独特的方式感知世界，但对感官感知变异的遗传基础知之甚少。在蝇眼中，R7感光受体亚型的随机嵌合是由转录因子Spineless （Ss）的随机开/关表达决定的。在一项全基因组关联研究中，我们在ss的调控DNA元件中发现了一个自然发生的插入，该插入降低了ss （ON）与ss （OFF）细胞的比例。这种光感受器命运的改变使果蝇天生的颜色偏好从绿色变成了蓝色。该遗传变异增加了对Klumpfuss （Klu）的结合亲和力，Klu是一种调节ss表达的锌指转录抑制因子。Klu在中间水平表达，以确定Ss（ON）与Ss（OFF）细胞的正常比例。因此，结合位点亲和性和转录因子水平被精细调节，以调节随机表达，设定不同命运的比例，并最终决定颜色偏好。
Each individual perceives the world in a unique way, but little is known about the genetic basis of variation in sensory perception. In the fly eye, the random mosaic of color detecting R7 photoreceptor subtypes is determined by stochastic on/off expression of the transcription factor Spineless (Ss). In a genome-wide association study, we identified a naturally occurring insertion in a regulatory DNA element in ss that lowers the ratio of Ss(ON) to Ss(OFF) cells. This change in photoreceptor fates shifts the innate color preference of flies from green to blue. The genetic variant increases the binding affinity for Klumpfuss (Klu), a zinc finger transcriptional repressor that regulates ss expression. Klu is expressed at intermediate levels to determine the normal ratio of Ss(ON) to Ss(OFF) cells. Thus, binding site affinity and transcription factor levels are finely tuned to regulate stochastic expression, setting the ratio of alternative fates and ultimately determining color preference.