INTRA-SEROGROUP AND INTER-SEROGROUP GENETIC RELATEDNESS OF ORBIVIRUSES .1. BLOT HYBRIDIZATION OF VIRUSES OF AUSTRALIAN SEROGROUPS
INTRA-SEROGROUP AND INTER-SEROGROUP GENETIC RELATEDNESS OF ORBIVIRUSES .1. BLOT HYBRIDIZATION OF VIRUSES OF AUSTRALIAN SEROGROUPS
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DOI：
10.1099/0022-1317-69-1-125
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发表时间：
1988-01-01
影响因子：
3.8
通讯作者：
KNUDSON, DL
中科院分区：
文献类型：
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作者：
GONZALEZ, HA;KNUDSON, DL
Orbiviruses 的 Eubenangee、Wallal 和 Warrego 血清群中的病毒主要在澳大利亚被分离出来。通过凝胶电泳和基因组 RNA 印迹杂交检查了这三个血清群中的几个分离株。通过同源基因之间的杂交程度来鉴定保守基因和变异基因。血清群内分离株的 dsRNA 图谱在聚丙烯酰胺凝胶中表现出异质性，但在琼脂糖凝胶中无法区分。血清群内的分离株通常在八个片段中表现出高水平的交叉杂交，其中同源片段2和6表现出杂交信号变异。尽管 Pata 病毒已被分类为 Eubenangee 血清群，但通过杂交，它与其他 Eubenangee 分离株的关系并不密切。同样，米切尔河病毒与瓦雷戈血清群的其他成员没有密切关系。应审查帕塔病毒和米切尔河病毒的分类地位，并在此期间将它们置于未分组的环状病毒组中。还通过印迹杂交检查了血清群间的相关性。来自欧本南吉的代表。对 Wallal、Warrego、蓝舌病、流行性出血性疾病和 Corriparta 血清群进行了检查。几个血清群关系较远，低水平的相关性表明有共同的祖先。虽然许多这些血清群可能在自然界中共同循环，但这些数据表明每个血清群代表一个独特的基因库。讨论了这些数据的分类学意义。
Viruses in the Eubenangee, Wallal and Warrego serogroups of orbiviruses have been isolated primarily in Australia. Several isolates in these three serogroups were examined by gel electrophoresis and blot hybridization of genomic RNA. Conserved and variant genes were identified by the degree of hybridization between cognate genes. The dsRNA profiles of isolates within a serogroup exhibited heterogeneity in polyacrylamide gels, but they were indistinguishable in agarose gels. Isolates within a serogroup generally showed a high level of cross-hybridization in eight segments with cognate segments 2 and 6 exhibiting hybridization signal variants. Although Pata virus has been classified in the Eubenangee serogroup, it was not closely related to other Eubenangee isolates by hybridization. Similarly, Mitchell River virus was not closely related to other members of the Warrego serogroup. The taxonomic status of Pata and Mitchell River viruses should be reviewed, and in the interim they should be placed in the ungrouped set of orbiviruses. Inter-serogroup relatedness was also examined by blot hybridization. Representatives from the Eubenangee. Wallal, Warrego, bluetongue, epizootic haemorrhagic disease and Corriparta serogroups were examined. Several of the serogroups were distantly related, and the low level of relatedness was suggestive of a common ancestry. While many of these serogroups may co-circulate in nature, these data suggest that each serogroup represents a distinct gene pool. The taxonomic significance of these data is discussed.