Isolation and native characterization of cysteine-rich collagens from bovine placental tissues and uterus and their relationship to types IV and V collagens

Isolation and native characterization of cysteine-rich collagens from bovine placental tissues and uterus and their relationship to types IV and V collagens
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牛胎盘组织和子宫中富含半胱氨酸的胶原蛋白的分离和天然表征及其与 IV 型和 V 型胶原蛋白的关系

DOI:
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发表时间:
1982
期刊:
影响因子:
4
通讯作者:
J. Weiss
J. Weiss
中科院分区:
生物学3区
文献类型:
--
作者:
M. Z. Abedin;S. Ayad;J. Weiss

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描述了一种从各种组织中分离和纯化不同类型胶原蛋白的简化方法,该方法在分离V型胶原蛋白和IV型胶原蛋白方面特别有效,并且高分子量。重量(HMW)从7S胶原聚集。子宫和母体绒毛含有两种形式的V型胶原-{α1(V)}2α2(V)和{α1(V)2α2(V)α3(V)}-已在DEAE-纤维素上分离。然而,子宫似乎是HMW聚集体和{α1(V)2α2(V)α3(V)}胶原蛋白的最丰富来源,并讨论了这些胶原蛋白之间的可能关系。
A simplified procedure for the fractionation and purification of different collagen types from various tissues is described which is particularly efficient in separating type-V from type-IV collagen, and highmol.-wt. (HMW) aggregates from 7 S collagen. Uterus and maternal villi contain 2 forms of type-V collagen -{α1(V)}2α2(V) and {α1(V)2α2(V)α3(V)}—which have been separated on DEAE-cellulose. Uterus however appears to be the richest source of both HMW aggregates and the {α1(V)2α2(V)α3(V)} collagen, and a probable relationship between these collagens is discussed.
人胎盘组织有限胃蛋白酶消化物中独特胶原成分的表征:天然聚集体的分子组织。
DOI: 10.1021/bi00509a035
发表时间: 1981
期刊: Biochemistry
影响因子: 2.9
作者:
Furuto,DK;Miller,EJ
通讯作者: Miller,EJ