Genome-Wide Analysis of Family I84 Protease Inhibitor Genes in Three Bivalves Reveals Important Information About the Protein Family's Evolution
Genome-Wide Analysis of Family I84 Protease Inhibitor Genes in Three Bivalves Reveals Important Information About the Protein Family's Evolution
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DOI：
10.1007/s10126-023-10236-1
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发表时间：
2023-08-14
影响因子：
3
通讯作者：
Xue，Qinggang
中科院分区：
文献类型：
--
作者：
Mao，Jinxia;Lu，Jiali;Xue，Qinggang
I84家族丝氨酸蛋白酶抑制剂被认为是软体动物特有的参与宿主防御的蛋白质。然而，该家族的分子进化仍有待了解。本研究在基因组水平上分析了长牡蛎、大西洋牡蛎和泥鳅3种双壳贝类中I84家族蛋白水解酶抑制因子的基因。共有66个家族I84基因(22个Inc.Gigas，28 Inc.维吉尼卡和16int.Granosa)是从3个种中鉴定的。它们在涉及4条染色体的基因组中分布不均。Gigasand 5染色体公司。维吉尼卡和T。Granosa和一些基因被随意复制。大多数基因有3个外显子，12个基因有4个外显子，1个基因有2个外显子。除1个基因外，其余基因均来自C。千兆和1FORM。在N端有一个信号序列，预测的成熟分子的性质是相似的。在66个氨基酸序列中确定了4个保守基序。共线分析表明，两种牡蛎在一般基因和I84家族基因上具有较高的共线性。对这66个基因与其他3个双壳类和1个腹足类的系统发育分析表明，同一物种的I84家族蛋白水解酶抑制因子基因在构建的最大似然树的末端分支中倾向于聚在一起，但大多数内部节点的Bootstrap值支持程度较差。此外，同一物种的基因在发育阶段和组织中的表达模式也存在差异。GigasandT.格兰诺萨。此外，还检测到家族I84基因和家族I84基因与非家族I84基因的共表达。GigasandT.格兰诺萨。这些结果表明，I84家族的蛋白水解酶抑制因子基因是在相关软体动物物种形成后通过活跃的复制和结构和功能的多样化而进化而来的，该多样化的蛋白水解酶抑制因子家族可能是多功能的。
Family I84 serine protease inhibitors are believed to be mollusk specific proteins involved in host defense. The molecular evolution of the family, however, remains to be understood. In this study, the genes of Family I84 protease inhibitors in 3 bivalves,Crassostrea gigas,Crassostrea virginicaandTegillarca granosa, were analyzed at the genomic level. A total of 66 Family I84 genes (22 inC. gigas, 28 inC. virginicaand 16 inT. granosa) were identified from the 3 species. They distributed unevenly in the genomes involving 4 chromosomes inC. gigasand 5 chromosomes inC. virginicaandT. granosaand some genes were tandemly duplicated. Most genes had 3 exons with 12 genes having 4 exons and 1 gene having 2 exons. All genes but 1 fromC. gigasand 1 fromT. granosaencoded peptides with a signal sequence at the N-terminus, and the properties of the predicted mature molecules were similar. Four conserved motifs were identified in the 66 amino acid sequences. Collinear analysis revealed higher collinearity between the 2 oyster species in general genes and in Family I84 genes. Phylogenetic analysis of the 66 genes with those previously reported from 3 other bivalves and 1 gastropod showed that Family I84 protease inhibitor genes from the same species tended to be grouped together in terminal branches of the constructed Maximum likelihood tree, but most internal nodes were poorly supported by the bootstrap values. In addition, differences in expression patterns between the genes of a same species were observed in the developmental stages and tissues ofC. gigasandT. granosa. Moreover, the co-expression of genes within Family I84 and Family I84 genes with non-Family I84 were also detected inC. gigasandT. granosa. These results suggested that Family I84 protease inhibitor genes evolved by active duplications and structural and functional diversifications after the speciation of related mollusks, and the diversified protease inhibitor family was likely multifunctional.