Immature human NT2 cells grafted into mouse brain differentiate into neuronal and glial cell types
Immature human NT2 cells grafted into mouse brain differentiate into neuronal and glial cell types
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DOI：
10.1016/s0014-5793(00)02251-1
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发表时间：
2000-12-08
期刊：
影响因子：
3.5
通讯作者：
Götz, J
中科院分区：
文献类型：
--
作者：
Ferrari, A;Ehler, E;Götz, J
NT 2细胞是一种可转染的人胚胎癌细胞系，其可分化为有丝分裂后神经元样细胞（NT 2N细胞），并可移植到啮齿动物脑中。分化需要在移植前用维甲酸进行为期5周的治疗。在这里，我们表明，这一步骤可以省略，和未分化的NT 2细胞迁移长距离和分化成神经元和少突胶质细胞样细胞类型后，移植到免疫活性的新生小鼠的大脑。移植的细胞可以被荧光金追踪，没有肿瘤形成的证据。我们的方法提供了一个实验模型系统，它允许在体内的人细胞的神经元和神经胶质分化的免疫组织学和生物化学研究，并可能适合作为一个体内模型的药理学研究。(C)2000年欧洲生物化学学会联合会。由Elsevier Science B.V.出版，版权所有。
NT2 cells are a transfectable human embryonal carcinoma cell line, that can be differentiated into postmitotic neuron-like cells (NT2N cells), and transplanted into rodent brains. Differentiation requires a 5-week-long treatment with retinoic acid prior to transplantation. Here, we show that this step can be omitted, and that undifferentiated NT2 cells migrate over long distances and differentiate into both neuron- and oligodendrocyte-like cell types upon grafting into brains of immunocompetent newborn mice. Grafted cells can be traced by fluorogold, with no evidence for tumor formation. Our approach provides an experimental model system which allows the immunohistological and biochemical study of neuronal and glial differentiation of human cells in vivo, and which may be suitable as an in vivo model for pharmacological studies. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.