miRNA-10a promotes cancer cell proliferation in oral squamous cell carcinoma by upregulating GLUT1 and promoting glucose metabolism
miRNA-10a promotes cancer cell proliferation in oral squamous cell carcinoma by upregulating GLUT1 and promoting glucose metabolism
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DOI：
10.3892/ol.2019.10257
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发表时间：
2019-06-01
期刊：
影响因子：
2.9
通讯作者：
Tong, Xin
中科院分区：
文献类型：
--
作者：
Chen, Yuan-Hua;Song, Yu;Tong, Xin
微小RNA-10a（miRNA-10a）促进肺癌；然而，据我们所知，它与其他癌症类型的关系尚不清楚。本研究旨在探讨 miRNA-10a 在口腔癌中的作用。采用逆转录定量聚合酶链反应检测口腔鳞状细胞癌（OSCC）患者肿瘤组织和癌旁健康组织中miRNA-10a和葡萄糖转运蛋白1（GLUT1）的表达水平。使用Pearson相关系数对miRNA-10a和GLUT1的表达水平进行相关分析。研究发现，与 OSCC 患者的邻近健康组织相比，miRNA-10a 和 GLUT1 在肿瘤组织中表达上调。 miRNA-10a和GLUT1的表达水平在肿瘤组织中呈正相关，但在邻近的健康组织中则不然。此外，miRNA-10a 过表达促进 OSCC 细胞中葡萄糖的摄取并上调 GLUT1。此外，GLUT1 过度表达促进葡萄糖摄取；然而，未检测到 OSCC 细胞中 miRNA-10a 表达水平的显着增加。 miRNA-10a和GLUT1的过度表达促进OSCC细胞增殖，而GLUT1敲低则抑制OSCC细胞增殖。 GLUT1 敲低还减弱了 miRNA-10a 过度表达对癌细胞增殖的增强作用。因此，miRNA-10a可能通过上调GLUT1和促进葡萄糖代谢来促进OSCC癌细胞增殖。
MicroRNA-10a (miRNA-10a) promotes lung cancer; however, to the best of our knowledge, its involvement in other cancer types is unknown. The present study aimed to investigate the role of miRNA-10a in oral cancer. Expression levels of miRNA-10a and glucose transporter 1 (GLUT1) in tumor tissues and adjacent healthy tissues obtained from patients with oral squamous cell carcinoma (OSCC) were detected by reverse transcription-quantitative polymerase chain reaction. Correlation analysis between the expression levels of miRNA-10a and GLUT1 was performed using Pearson's correlation coefficient. It was identified that miRNA-10a and GLUT1 were upregulated in tumor tissues compared with adjacent healthy tissues of patients with OSCC. Expression levels of miRNA-10a and GLUT1 were positively correlated in tumor tissues but not in adjacent healthy tissues. In addition, miRNA-10a overexpression promoted glucose uptake and upregulated GLUT1 in OSCC cells. Furthermore, GLUT1 overexpression promoted glucose uptake; however, no significant increase in the expression level of miRNA-10a in OSCC cells was detected. Overexpression of miRNA-10a and GLUT1 promoted OSCC cell proliferation, while GLUT1-knockdown inhibited OSCC cell proliferation. GLUT1-knockdown also attenuated the enhancing effect of miRNA-10a overexpression on cancer cell proliferation. Therefore, miRNA-10a may promote cancer cell proliferation in OSCC by upregulating GLUT1 and promoting glucose metabolism.