Surface-directed capillary system; theory, experiments and applications
Surface-directed capillary system; theory, experiments and applications
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DOI：
10.1039/b502207j
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发表时间：
2005-01-01
期刊：
影响因子：
6.1
通讯作者：
Jonsmann, J
中科院分区：
文献类型：
--
作者：
Bouaidat, S;Hansen, O;Jonsmann, J
我们提出了一种用于微系统中液体输送的毛细管流系统。我们的简单微流控系统由两个平行的平面组成，用间隔片隔开。其中一个表面是完全疏水的，另一个主要是疏水的，但通过光刻技术在其上定义了亲水途径。通过以这种方式控制表面的润湿特性，液体可以被限制在由亲水性途径定义的某些区域。这种技术消除了对两个表面对齐的需要。图案等离子体聚合的六氟丙烯构成疏水区域，而未经处理的玻璃表面构成亲水通道。建立了毛细管流动的理论模型，得到了与实验结果吻合较好的解析解。毛细管驱动的微流系统还用于在平面底物上对生物材料进行定形和固定：使用毛细管流系统制备了定义明确的200 μ m宽的人细胞（HeLa）条和荧光标记蛋白（荧光素异硫氰酸酯标记的牛血清白蛋白，即FITC-BSA）。
We present a capillary flow system for liquid transport in microsystems. Our simple microfluidic system consists of two planar parallel surfaces, separated by spacers. One of the surfaces is entirely hydrophobic, the other mainly hydrophobic, but with hydrophilic pathways defined on it by photolithographic means. By controlling the wetting properties of the surfaces in this manner, the liquid can be confined to certain areas defined by the hydrophilic pathways. This technique eliminates the need for alignment of the two surfaces. Patterned plasma-polymerized hexafluoropropene constitutes the hydrophobic areas, whereas the untreated glass surface constitutes the hydrophilic pathways. We developed a theoretical model of the capillary flow and obtained analytical solutions which are in good agreement with the experimental results. The capillarity-driven microflow system was also used to pattern and immobilize biological material on planar substrates: well-defined 200 mu m wide strips of human cells ( HeLa) and fluorescence labelled proteins ( fluorescein isothiocyanate-labelled bovine serum albumin, i.e., FITC-BSA) were fabricated using the capillary flow system presented here.