Understanding the role of water in the aggregation of proteins and polymers in aqueous solution using near-infrared spectroscopy
Understanding the role of water in the aggregation of proteins and polymers in aqueous solution using near-infrared spectroscopy
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使用近红外光谱了解水在水溶液中蛋白质和聚合物聚集中的作用
DOI：
10.1177/0960336020944766
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发表时间：
2020-07
期刊：
影响因子：
--
通讯作者：
Xueguang Shao
中科院分区：
文献类型：
--
作者：
Yan Sun;Li Ma;Li Wang;Xuewei Zhu;Wensheng Cai;Xueguang Shao
水在化学和生物过程中起着重要作用。为了了解水在蛋白质和聚合物聚集过程中的作用，利用近红外光谱研究了水在蛋白质和聚合物聚集过程中的结构变化。测定了不同浓度的R2/wt和聚（N，N-二甲氨基乙基甲基丙烯酸酯）水溶液在不同温度下的近红外光谱。利用化学计量学方法分析了溶质和水的光谱随温度的变化。在R2/wt的聚集过程中，在NH基团周围有一个氢键的水分子解离，引发水化水氢键网络的改变，然后，在疏水侧链附近有两个氢键（S2）的水分子从R2/wt中释放出来，从而形成有序淀粉样纤维。在低浓度聚（N，N-二甲氨基乙基甲基丙烯酸酯）溶液的聚集过程中，聚合物的链在36℃以下倾向于形成松散的疏水结构，然后在较低的临界溶液温度39℃左右聚集成胶束。S2在松散的疏水结构中充当连接聚合物链的桥梁，S2桥在高温下的解离是胶束形成的原因。而在高浓度溶液中，在聚集体中没有发现S2的光谱信息，这表明脱水链直接形成了胶束。
Water plays an important role in chemical and biological processes. For understanding the role of water in the aggregation of proteins and polymers, the variation of water structures in the process of aggregation was studied by near-infrared spectroscopy. The near-infrared spectra of the aqueous R2/wt and poly(N,N-dimethylaminoethyl methacrylate) solutions of different concentrations were measured at different temperatures. The spectral changes of the solutes and water with temperature were analyzed with the help of chemometric methods. In the aggregation of R2/wt, the water species with one hydrogen bond around the NH groups dissociate to initiate the change of the hydrogen bonding network of the hydration water, and then, the water molecules with two hydrogen bonds (S2) near the hydrophobic side chains release from the R2/wt, resulting in the formation of the ordered amyloid fibers. In the aggregation process of low concentration poly(N,N-dimethylaminoethyl methacrylate) solutions, the chains of the polymer tend to form a loose hydrophobic structure below 36°C and then aggregate into a micelle at a lower critical solution temperature of around 39°C. S2 acts as a bridge to connect the polymer chains in the loose hydrophobic structure, and the dissociation of the S2 bridge at high temperature is the reason for the formation of the micelle. For high concentration solution, however, the spectral information of S2 was not found in the aggregation, suggesting a direct formation of the micelle from the dehydrated chains.
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影响因子：
2.8
作者：
D. Zhong;S. Pal;A. Zewail
通讯作者：
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DOI：
10.1016/j.saa.2020.118046
发表时间：
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期刊：
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
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DOI：
10.1016/j.chemolab.2018.10.006
发表时间：
2018-12-15
影响因子：
3.9
作者：
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影响因子：
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作者：
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